在pH 为7.41的生理条件下，以溴化乙锭（EB）作为荧光探针，通过紫外可见光谱法(UV-Vis)和荧光光谱法对茜草色素类食用色素蒽棕（Ant）与鲱鱼精脱氧核糖核酸(DNA)分子之间的相互作用机理做了初步探讨。通过循环伏安法研究蒽棕在玻碳电极上的电化学规律，根据蒽棕与DNA作用的循环伏安曲线及EB对Ant与DNA作用的影响，推断Ant与DNA 相互作用的主要方式为嵌插。结果显示：蒽棕与DNA 分子之间相互作用的结合比为n(Ant):n(DNA)=3.68:1，25 ℃时的结合常数K=2.084×103 L/mol，Ant-DNA 复合物的表观摩尔吸光系数ε=2.21×104 L·mol-1·cm-1。探讨了盐效应对蒽棕与DNA分子相互作用的影响以及它们之间的作用方式，发现蒽棕电化学变化规律同EB相似，这些实验结果证明茜草类食用色素中的蒽棕能够与DNA 分子发生相互作用，相互作用方式存在嵌插。

Under the physiological condition that pH is 7.41, the mechanism of interactions between anthragallol (Ant) and deoxyribonucleic acid (DNA) is investigated by ultraviolet- visible spectroscopy and fluorescence spectroscopy using ethidium bromide (EB) as probe. The electrochemical characterization of Ant on glassy carbon electrode is determined by cyclic voltammetry. Based on the curves of cyclic voltammetry and the effect of EB on the interactions of Ant with DNA, it is proposed that the binding of Ant to DNA conforms to an intercalative mode. The results show that the binding ratio of Ant and DNA is 3.68:1, the binding constant (K) of Ant with DNA is 2.084×103 L/mol at 25 oC and the apparent molar absorption coefficient (e) is 2.21×104 L·mol-1·cm-1. The influence of salt effect on the interactions of Ant with DNA is studied, and the electrochemical changing rule of Ant is similar to EB with DNA. The results also show that Ant can bind with DNA, intercalation binding is the binding mode between Ant and DNA.