光学学报, 2015, 35 (4): 0409001, 网络出版: 2015-02-02
去卷积数字全息重构的微球位置精确测量
Deconvolution in Digital Holographic Reconstruction for High-Accuracy Position of Microsphere Tracking Technology
测量 数字全息 重构 微球三维位置 去卷积 单分子力谱 measurement digital holography reconstruction three-dimensional position of microspheres deconvolution single molecule force spectrum
摘要
微球三维位置的精确测量是单分子力谱测量技术中的关键。采用同轴数字全息技术对微球的三维位置进行测量。通过同轴数字全息显微系统采集一系列微球的全息图像,利用瑞利-索末菲传播原理对全息图进行三维重构,同时对重构光场进行去卷积运算,消除了散斑、离焦信号等噪音,并对微球球心纵向光场分布进行多项式拟合,提高了微球三维位置测量精度。实验表明,该方法不仅能够对全视场中的微球进行并行测量,而且能够对交叠微球进行测量,纵向分辨力达到2 nm,在生物单分子动力学、粒子图像测速技术等研究领域具有重要意义。
Abstract
High resolution three-dimensional (3D) position of microspheres tracking is the key point in the aero of single molecule force spectrum. Digital holography is emerging as a promising optical technique for 3D microspheres tracking. A sequence of holograms are acquired using charge couple device with an in-line digital holographic microscopy. Each hologram is subsequently reconstructed with Rayleigh-Sommerfeld propagation function and then deconvolved it with its propagator to suppress the artifacts and blur. Also polynomial fitting is introduced to fit the intensity profile of the volumetric reconstruction in the Z direction which can improve the resolution of 3D microspheres tracking. As a result, this method can measure multiple microspheres simultaneously and also can measure the overlapped spheres. The resolution in the Z direction is approach 2 nm, this is significant to the study of single biological molecule dynamics and particles image velocimetry etc.
雷海, 常新宇, 谢湘军, 曾雅楠, 胡晓东, 胡小唐. 去卷积数字全息重构的微球位置精确测量[J]. 光学学报, 2015, 35(4): 0409001. Lei Hai, Chang Xinyu, Xie Xiangjun, Zeng Yanan, Hu Xiaodong, Hu Xiaotang. Deconvolution in Digital Holographic Reconstruction for High-Accuracy Position of Microsphere Tracking Technology[J]. Acta Optica Sinica, 2015, 35(4): 0409001.