首页 > 论文 > 光子学报 > 44卷 > 2期(pp:217002--1)

快速光脉冲藻类光合作用测量方法的激发条件研究

Determining the Optimal Excitation Condition of High-frequency Flash Method for Algae Photosynthetic Parameters Measurement

  • 摘要
  • 论文信息
  • 参考文献
  • 被引情况
  • PDF全文
分享:

摘要

激发光强和激发持续时间是快速光脉冲藻类光合作用测量方法的关键实验条件.通过光脉冲激发实验, 定量分析了不同平均激发光强下还原态初级电子受体的比例和发生再氧化的初级电子受体的比例.结果表明:快速光脉冲激发的最佳平均光强为30 000 μmol quanta·m-2·s-1, 最佳激发持续时间为70 μs;30 000 μmol quanta·m-2·s-1平均激发光强能够在70 μs内还原96.08%的初级电子受体, 且仅9.81%的初级电子受体发生了再氧化.

Abstract

The excitation intensity and excitation duration are the essential experimental conditions of the high-frequency flash method for the measurement of algae photosynthetic parameters. The fraction of reduced primary electron acceptors and the reoxidation extent of these reduced primary electron acceptors were analyzed under different excitation intensities and excitation durations. The analysis results indicate that 30 000 μmol quanta·m-2·s-1 is the optimal excitation intensity and 70 μs is the optimal excitation duration. Under this optimal excitation condition, 96.08% of the primary electron acceptors are reduced and only 9.81% primary electron acceptors reoxidize.

广告组1 - 空间光调制器+DMD
补充资料

中图分类号:X122

DOI:10.3788/gzxb20154402.0217002

所属栏目:医用光学与生物技术

基金项目:The Natural Science Foundation of China(No. 31400317), Natural Science Foundation of Anhui Province (No. 1408085MD72), National “863” Program of China (No.2014AA06A509), Science and Technology Planning Project of Anhui Province (No. 1206c0805012), National “863” Program of China (Nos. 2013AA065502, 2009AA063005), National Natural Science Foundation of China (Project No. 61378041), and Excellent Youth Foundation of Anhui Scientific Committee (No. 1108085J19)

收稿日期:2014-08-24

修改稿日期:2014-09-30

网络出版日期:--

作者单位    点击查看

石朝毅:中国科学院安徽光学精密机械研究所 国家环境光学监测技术重点实验室, 合肥 230031
张玉钧:中国科学院安徽光学精密机械研究所 国家环境光学监测技术重点实验室, 合肥 230031
殷高方:中国科学院安徽光学精密机械研究所 国家环境光学监测技术重点实验室, 合肥 230031
赵南京:中国科学院安徽光学精密机械研究所 国家环境光学监测技术重点实验室, 合肥 230031
段静波:中国科学院安徽光学精密机械研究所 国家环境光学监测技术重点实验室, 合肥 230031
邱晓晗:中国科学院安徽光学精密机械研究所 国家环境光学监测技术重点实验室, 合肥 230031
方丽:中国科学院安徽光学精密机械研究所 国家环境光学监测技术重点实验室, 合肥 230031
肖雪:中国科学院安徽光学精密机械研究所 国家环境光学监测技术重点实验室, 合肥 230031
刘文清:中国科学院安徽光学精密机械研究所 国家环境光学监测技术重点实验室, 合肥 230031

联系人作者:SHIChao-yi(cyshi@aiofm.ac.cn)

备注:SHI Chao-yi (1986-), male, Ph.D. candidate, mainly focuses on photoelectric information processing.

【1】HAN Bo-ping, HAN Zhi-guo, FU Xiang. Algae photosynthesis: mechanism and model[M].Beijing: Science Press, 2003.

【2】SUGGETT D J, PRIL O, BOROWITZKA M A, BOROWITZKA, et al. Chlorophyll a fluorescence in aquatic sciences: methods and applications[M].Springer, 2011.

【3】PAPAGEORGIOU G C, TSIMILLI-MICHAEL M, STAMATAKIS K. The fast and slow kinetics of chlorophyll a fluorescence induction in plants, algae and cyanobacteria: a viewpoint[J].Photosynthesis Research, 2007, 94(2-3): 275-290.

【4】SERDIO J. A chlorophyll fluorescence index to estimate short-term rates of photosynthesis by intertidal microphytobenthos[J].Journal of Phycology, 2003, 39(1): 33-46.

【5】BAKER N R. Chlorophyll fluorescence: A probe of photosynthesis in vivo[J].Annual Review of Plant Biology, 2008, 59: 89-113.

【6】MAUZERALL D. Light-induced fluorescence changes in Chlorella, and the primary photoreactions for the production of oxygen[J].Proceedings of the National Academy of Sciences, 1972, 69(6): 1358-1362.

【7】SCHREIBER U. Detection of rapid induction kinetics with a new type of high-frequency modulated chlorophyll fluorometer[M].Current topics in photosynthesis. Springer Netherlands, 1986: 259-270.

【8】KOLBER Z S, PRIL O, FALKOWSKI P G. Measurements of variable chlorophyll fluorescence using fast repetition rate techniques: defining methodology and experimental protocols[J].Biochimica et Biophysica Acta (BBA)-Bioenergetics, 1998, 1367(1): 88-106.

【9】FALKOWSKI P G, KOLBER Z S. Fast repetition rate (FRR) fluorometer and method for measuring fluorescence and photosynthetic parameters: U S 5426306[P].1995-06-20.

【10】SHI Chao-yi, ZHANG Yu-jun, YIN Gao-fang, et al. Measurement of algae PSII photosynthetic parameters using high-frequency excitation flashes[J].Chinese Optics Letters, 2014, 12(8): 080101.

【11】KOLBER Z S, FALKOWSKI P G. Multiple protocol fluorometer and method: US,6121053[P].2000-9-19.

【12】FALKOWSKI P, KIEFER D A. Chlorophyll a fluorescence in phytoplankton: relationship to photosynthesis and biomass[J].Journal of Plankton Research, 1985, 7(5): 715-731.

【13】BISWAL A K, DILNAWAZ F, DAVID K A V, et al. Increase in the intensity of thermoluminescence Q-band during leaf ageing is due to a block in the electron transfer from QA to QB[J].Luminescence, 2001, 16(5): 309-313.

【14】VREDENBERG W, PRIL O. On the polyphasic quenching kinetics of chlorophyll a fluorescence in algae after light pulses of variable length[J].Photosynthesis Research, 2013, 117(1-3): 321-337.

【15】FALKOWSKI P, KIEFER D A. Chlorophyll a fluorescence in phytoplankton: relationship to photosynthesis and biomass[J].Journal of Plankton Research, 1985, 7(5): 715-731.

【16】BELYAEVA N E, SCHMITT F J, STEFFEN R, et al. PS II model-based simulations of single turnover flash-induced transients of fluorescence yield monitored within the time domain of 100 ns-10 s on dark-adapted Chlorella pyrenoidosa cells[J].Photosynthesis Research, 2008, 98(1-3): 105-119.

【17】WAN Wen-bo, HUA Deng-xin. Laser-induced chlorophyll fluorescANce lifetime measurement and characteristic analysis[J].Acta Physica Sinica, 2013, 62(19).

【18】LIU Jing, LIU Wen-qing, ZHAO Nan-jing, et al. Phytoplankton chlorophyll fluorescence characteristics excited by various light qualities and intensities [J].Acta Optica Sinica, 2013, 09: 290-297.

【19】SOMMEVELD A, RADEMAKER H, DUYSENS L N M. Transfer and trapping of excitation energy in Photosystem II as studied by chlorophyll a fluorescence quenching by dinitrobenzene and carotenoid triplet. The matrix model[J].Biochimica et Biophysica Acta (BBA)-Bioenergetics, 1980, 593(2): 272-289.

【20】DEPREZ J, DOBEK A, GEACINTOV N E, et al. Probing fluorescence induction in chloroplasts on a nanosecond time scale utilizing picosecond laser pulse pairs[J].Biochimica et Biophysica Acta (BBA)-Bioenergetics, 1983, 725(3): 444-454.

引用该论文

SHI Chao-yi,ZHANG Yu-jun,YIN Gao-fang,ZHAO Nan-jing,DUAN Jing-bo,QIU Xiao-han,FANG Li,XIAO Xue,LIU Wen-qing. Determining the Optimal Excitation Condition of High-frequency Flash Method for Algae Photosynthetic Parameters Measurement[J]. ACTA PHOTONICA SINICA, 2015, 44(2): 0217002

石朝毅,张玉钧,殷高方,赵南京,段静波,邱晓晗,方丽,肖雪,刘文清. 快速光脉冲藻类光合作用测量方法的激发条件研究[J]. 光子学报, 2015, 44(2): 0217002

被引情况

【1】石朝毅,高先和,殷高方,周泽华,卢军,胡学友. 基于可变光脉冲诱导荧光的浮游植物光合作用参数测量系统设计. 激光与光电子学进展, 2016, 53(7): 72301--1

【2】王翔,殷高方,赵南京,甘婷婷,覃志松,陈敏,华卉,董鸣,丁志超,亓培龙,王璐,孟德硕,刘建国. 基于TPLIF技术的光合荧光参数快速准确测量的激发光强自适应方法. 光学学报, 2020, 40(24): 2412001--1

您的浏览器不支持PDF插件,请使用最新的(Chrome/Fire Fox等)浏览器.或者您还可以点击此处下载该论文PDF