摘要

激发光强和激发持续时间是快速光脉冲藻类光合作用测量方法的关键实验条件.通过光脉冲激发实验, 定量分析了不同平均激发光强下还原态初级电子受体的比例和发生再氧化的初级电子受体的比例.结果表明：快速光脉冲激发的最佳平均光强为30 000 μmol quanta·m-2·s-1, 最佳激发持续时间为70 μs;30 000 μmol quanta·m-2·s-1平均激发光强能够在70 μs内还原96.08%的初级电子受体, 且仅9.81%的初级电子受体发生了再氧化.

Abstract

The excitation intensity and excitation duration are the essential experimental conditions of the high-frequency flash method for the measurement of algae photosynthetic parameters. The fraction of reduced primary electron acceptors and the reoxidation extent of these reduced primary electron acceptors were analyzed under different excitation intensities and excitation durations. The analysis results indicate that 30 000 μmol quanta·m-2·s-1 is the optimal excitation intensity and 70 μs is the optimal excitation duration. Under this optimal excitation condition, 96.08% of the primary electron acceptors are reduced and only 9.81% primary electron acceptors reoxidize.

补充资料

中图分类号：X122

DOI：10.3788/gzxb20154402.0217002

所属栏目：医用光学与生物技术

基金项目：The Natural Science Foundation of China(No. 31400317), Natural Science Foundation of Anhui Province (No. 1408085MD72), National “863” Program of China (No.2014AA06A509), Science and Technology Planning Project of Anhui Province (No. 1206c0805012), National “863” Program of China (Nos. 2013AA065502, 2009AA063005), National Natural Science Foundation of China (Project No. 61378041), and Excellent Youth Foundation of Anhui Scientific Committee (No. 1108085J19)

收稿日期：2014-08-24

修改稿日期：2014-09-30

网络出版日期：--

作者单位    点击查看

联系人作者：SHIChao-yi(cyshi@aiofm.ac.cn)

备注：SHI Chao-yi (1986-), male, Ph.D. candidate, mainly focuses on photoelectric information processing.

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引用该论文

SHI Chao-yi,ZHANG Yu-jun,YIN Gao-fang,ZHAO Nan-jing,DUAN Jing-bo,QIU Xiao-han,FANG Li,XIAO Xue,LIU Wen-qing. Determining the Optimal Excitation Condition of High-frequency Flash Method for Algae Photosynthetic Parameters Measurement[J]. ACTA PHOTONICA SINICA, 2015, 44(2): 0217002

石朝毅,张玉钧,殷高方,赵南京,段静波,邱晓晗,方丽,肖雪,刘文清. 快速光脉冲藻类光合作用测量方法的激发条件研究[J]. 光子学报, 2015, 44(2): 0217002

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