为了实现实时数字全息显微观测,采用数字全息并行算法达到实时再现要求。首先根据设计的数字全息显微镜光路结构,充分利用图像传感器空间带宽积,通过实时记录方案采集全息图、物光强及参考光强以消除0级;然后通过设计的并行再现软件,将采集到的图像均匀分割为4个区域,交由4个进程分别同时计算,每个进程实现对应区域的全息图再现后,将每个再现结果再均分成4个区域,并将对应区域重新组合成4组数据交由4个进程分别同时进行叠加,计算相位及强度;最后将4个进程计算得到的相位、强度重新拼接成完整的再现强度及相位图。结果表明,系统的数据采集和图像再现速度达到了18frame/s。该设计系统实现了实时全息显微观测。

For realtime measurement of digital holographic microscopy (DHM), a parallel algorithm was proposed. Firstly, realtime recording strategy was adopted to record hologram, intensity of object wave and intensity of reference wave according to light path of DHM. Zeroorder of hologram was removed by this strategy. So, the product of spatial bandwidth of image sensor was efficiently utilized. Then, parallel reconstructing software was developed. The captured image was separated to four partitions and was sent to four procedures which run simultaneously. After the partitions were reconstructed by the corresponding procedure, each reconstructed result was separated to four regions. The corresponding regions were combined to four groups and were sent to four procedures for superposing and calculating phase and intensity. Finally, the whole phase and intensity map were obtained by merging four regions of phase and intensity. The experimental results reveal that the speed for image capture and hologram reconstruction is up to 18frame/s. Realtime measurement is realized with the proposed system.