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胃癌血清的表面增强拉曼光谱研究

The Study of Gastric Cancer Serum on Surface Enhanced Raman Spectroscopy Technology

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摘要

表面增强拉曼光谱技术在癌症诊断领域已经有了广泛的研究和应用,本文利用金纳米溶胶为增强基底,采用便携式近红外拉曼光谱系统,对89例胃癌患者及正常人血清样本进行了SERS光谱探测.结果表明,血清的特征峰主要归属于氨基酸,其次是核酸、糖类及脂类.相比于正常人血清SERS光谱,胃癌血清中归属于核酸的特征峰强度都较高,大部分归属于蛋白质的特征峰强度较低,与医学研究结论相符.说明SERS技术可以有效地反映胃癌患者和正常人血清的差异,为后期SERS技术快速诊断胃癌提供了实验基础.

Abstract

Surface-enhanced Raman spectroscopy has been extensively studied and applied in cancer diagnosis field.In this paper,gold colloid was prepared as SERS substrate and its enhanced factor was calculated using crystal violet molecules as probes,and the SERS spectra of serum of gastric cancer patients and healthy people were detected.The enhancement effect of SERS spectra of serum using gold colloid as SERS substrate was significant comparing with Raman spectra of serum.The Raman peaks were mainly assigned to amino acid,nucleic acid,and carbohydrate etc.Comparing with the healthy people,the Raman intensities of nucleic acids of gastric cancer patients were higher and that of proteins were lower,which coincided with the conclusion of medical researches.It indicates that SERS is suitable for distinguishing gastric carcinoma and provides a promising approach for gastric cancer diagnosis.

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中图分类号:O657.37;R730.57

DOI:10.3969/j.issn.1007-7146.2015.01.010

所属栏目:基础研究

基金项目:山东省自然科学基金项目(BS2013HZ006);青岛市科技计划基础研究项目(12-1-4-1-(1)-jch,14-2-4-96-jch)

收稿日期:2014-10-13

修改稿日期:2014-12-22

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作者单位    点击查看

孟辰:中国海洋大学光学光电子青岛市重点实验室, 山东 青岛 266100
周涵婧:中国海洋大学光学光电子青岛市重点实验室, 山东 青岛 266100
史晓凤:中国海洋大学光学光电子青岛市重点实验室, 山东 青岛 266100
毛伟征:青岛大学医学院青岛市立医院普外科, 山东 青岛 266071
马君:中国海洋大学光学光电子青岛市重点实验室, 山东 青岛 266100

联系人作者:孟辰(tmcing@qq.com)

备注:孟辰(1990-),男,山东济宁人,硕士研究生,主要从事光谱技术及其应用方面的研究.

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引用该论文

MENG Chen,ZHOU Hanjing,SHI Xiaofeng,MAO Weizheng,MA Jun. The Study of Gastric Cancer Serum on Surface Enhanced Raman Spectroscopy Technology[J]. Acta Laser Biology Sinica, 2015, 24(1): 68-72

孟辰,周涵婧,史晓凤,毛伟征,马君. 胃癌血清的表面增强拉曼光谱研究[J]. 激光生物学报, 2015, 24(1): 68-72

被引情况

【1】刘健,郝倩云,李云涛. 金-银合金纳米溶胶对血清SERS增强活性研究. 应用激光, 2017, 37(5): 722-726

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