采用拉曼和UV光谱研究了抗癌药物4-硫代胸腺嘧和4-硫胸腺嘧啶脱氧核苷的特征结构,确定了药物在酸性、中性和碱性环境下的结构变化.研究结果表明,在酸性和中性溶液中,4-硫代胸腺嘧和4-硫胸腺嘧啶脱氧核苷被强烈质子化,而在碱性环境中则是去质子化,同时,4-硫胸腺嘧啶脱氧核苷的质子化和去质子化比4-硫代胸腺嘧弱,说明氢键的结合对4-硫代胸腺嘧的NH质子和溶液分子之间的相互作用具有重要影响.

Raman and Ultraviolet spectroscopies were used to analyze the characteristic structures of both anti-tumor drug 4-thiothymine and 4-thiothymidine and to determine the structural changes under acidic,neutral and alkaline conditions in this paper.The results show that 4-thiothymine and 4-thiothymidine are protonated strongly when being titrated into either basic or acidic solution,and are deprotonated in the alkaline environments,but the protonation and deprotonation of 4-thiothymidine is weaker than that of 4-thiothymine.It indicates that the hydrogen-bonding has an important effect on interaction between the NH proton of the 4-thiothymine base and the solvent molecules.