本文在研究了离体人体鼻咽正常组织和癌变组织的荧光寿命的基础上, 实验研究了生理盐水的浓度、组织光学特性参数、激发光源的偏振性对癌变和正常鼻咽组织的荧光寿命的影响。实验结果表明: 组织的光学特性参数对组织的荧光寿命有不同程度的影响; 而不同浓度的生理盐水和光源的偏振性对组织的荧光寿命没有显著的影响。荧光寿命与该发射荧光的强度没有关系, 只决定于局部环境, 受微环境的物理化学性质因素的影响, 因此荧光寿命作为人体组织癌变的检测方法, 有着很好的应用前景。

The life-times of normal nasopharyngeal tissues and nasopharyngeal carcinoma tissues were determined. Furthermore, impact including concentration of brine, optical tissue properties, polarization of excitation light source on auto-fluorescence lifetime of human nasopharyngeal carcinoma and normal nasopharyngeal tissues in vitro were investigated, respectively. The results show that optical tissue properties have different effects on fluorescence lifetimes of nasopharyngeal tissues, while concentration of brine and polarization of excitation light source have no significant influence on fluorescence lifetime. Fluorescence lifetime is determined by physical and chemical properties of microenvironment and has nothing to do with fluorescence intensity, it can be used as a testing method for tumor.