目的: 探讨布美他尼对PDT诱导加剧的大鼠C6胶质瘤瘤周水肿的治疗作用。方法: 培养C6胶质瘤细胞, 建立C6大鼠胶质瘤模型, 分成: A: 空白对照组, B: 光动力治疗组, C: 布美他尼治疗组, D: 光动力和布美他尼联合治疗组。荷瘤21天后取材测量瘤重, 瘤周水含量, 微血管密度, NKCC-1和ZO-1的表达。另外MRI监测肿瘤生长, 记录大鼠生存时间。结果: PDT能够抑制胶质瘤细胞生长增殖, 杀伤肿瘤细胞, 促使肿瘤细胞凋亡, 下调紧密连接相关蛋白的表达来打开紧密连接, 从而延长生存期, 同时诱使NKCC-1过表达引起瘤周水肿加剧。结论: 应用布美他尼联合治疗能抑制PDT单独应用引起的NKCC-1过表达, 减轻PDT加重的瘤周水肿, 增强PDT的杀肿瘤作用, 而不减少ZO-1的表达。

Objective: To investigate the effect of bumetanide on peri- tumor edema caused by photodynamic therapy (PDT) of intraparenchymal C6 glioma xenografts. Methods: Seven days after inoculation with C6 cells, rats with MRI-confirmed glioma received PDT, injection of bumetanide or a combination of the two treatments. After treatment, tumor volume, tumor weight, brain water content, microvessel density, expression of NKCC-1, ZO-1 and animal survival time were examined. Results: In the PDT group, tumor growth was significantly inhibited and survival prolonged. Bumetanide enhanced the efficacy of PDT and reduced PDT-induced peri-tumor edema in the combined PDT+bumetanide treatment group where NKCC-1 expression in response to PDT was significantly suppressed. ZO-1 expression was significantly suppressed in the PDT-only group. This suppression was not observed in the combined PDT+bumetanide treatment group. Conclusion: PDT, in combination with bumetanide was observed to significantly inhibit the growth of C6 glioma, relieve peri-tumor edema caused by PDT alone and prolongsurvival. These results suggest that PDT, in combination with bumetanide, may be a useful and promising strategy in the treatment of human glioma.