激光生物学报, 2014, 23 (5): 417, 网络出版: 2015-07-20
He-Ne激光对啤酒大麦麦芽同工酶基因表达的影响
Effects of He-Ne Laser on the Isozyme Expression of Beer Barley
摘要
采用不同时间He-Ne激光(5 mW/mm2)对“临啤二号”大麦种子进行辐照, 利用聚丙烯酰胺凝胶电泳(PAGE)技术分析激光辐照对大麦麦芽中超氧化物歧化酶(SOD)同工酶、过氧化物(POD)同工酶、过氧化氢(CAT)同工酶、β-淀粉酶同工酶、三磷酸腺苷(ATPase)同工酶酶谱的影响。结果表明: 种子经He-Ne激光辐照后诱导出1条新POD同工酶谱带, 且总POD活性在60 s和90 s处有所增加; 30 s激光辐照诱导出1条新的CAT同工酶谱带, 其它时间处理酶的组分没有变化, 但酶活性增加; He-Ne激光辐照使SOD、β-淀粉酶及ATPase同工酶的组分在不同时间下有所减少, 但一定时间的激光辐照提高了其同工酶的活性。综合实验结果可知, 60 s~90 s He-Ne激光可以促进大麦种子萌发过程中以上同工酶基因的表达, 有利于缩短制麦周期, 提高麦芽糖化力及抗氧化力。
Abstract
The seeds of a beer barley cultivar (Linpi No.2) were exposed to He-Ne laser irradiation (5 mW/mm2) with varied duration, the polyacrylamide gel electrophoresis (PAGE) was used to analyze the patters of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), β-amylase and adenosine triphosphatase (ATPase) in malt. The results showed that one new band of POD zymogram appeared and the activities of total POD enhanced in 60 s and 90 s radiation groups after He-Ne laser irradiation compared with the control; as for CAT, it showed that one new band of CAT zymogram appeared in 30 s radiation group and the activities of total CAT enhanced though there are no isozyme bands changing in other groups; there were isozyme bands disappearing in SOD, β-amylase and ATPase in different radiation groups compared to that of control, but the activities of the isozymes increased after certain duration radiation. To sum up, 60 s ~90 s irradiation promoted the expression of above isozyme genes which might shorten the malting process and improve the malt diastatic power and antioxidant capacity.
闫蓉, 虞波, 李娜, 薛燕燕, 赵岩, 韩榕, 杨利艳. He-Ne激光对啤酒大麦麦芽同工酶基因表达的影响[J]. 激光生物学报, 2014, 23(5): 417. YAN Rong, YU Bo, LI Na, XUE Yanyan, ZHAO Yan, HAN Rong, YANG Liyan. Effects of He-Ne Laser on the Isozyme Expression of Beer Barley[J]. Acta Laser Biology Sinica, 2014, 23(5): 417.