目的: 探讨658 nm低能量激光照射对人牙周膜细胞增殖、碱性磷酸酶活性及纤维连接蛋白合成的影响。方法: 改良组织块法体外培养人牙周膜细胞。通过658 nm激光照射人牙周膜细胞, 观察能量密度为1.86 J/cm2和3.72 J/cm2激光照射后不同时间点细胞增殖效应、碱性磷酸酶活性和纤维连接蛋白的变化。结果: 1.86 J/cm2和3.72 J/cm2能量密度的激光照射人牙周膜细胞, 可显著促进细胞增殖效应。3.72 J/cm2能量密度的激光照射可提高人牙周膜细胞碱性磷酸酶活性;能量密度为1.86 J/cm2的激光照射人牙周膜细胞72 h后, 细胞中纤维连接蛋白分泌量增加。结论: 658 nm低剂量激光照射可促进人牙周膜细胞增殖; 适量的低剂量激光照射人牙周膜细胞可促进其碱性磷酸酶活性及纤维连接蛋白的分泌。

Objective: To investigate the effects of low-level 658 nm laser irradiation on the proliferation,alkaline phosphatase activity and fibronection of human periodontal ligament cells(hPDLCs). Methods: hPDLCs obtained from premolar periodontal ligaments were cultured according to the method of modified tissue culture.hPDLCs were subcultured on 96-well tissue culture plates and cultured for 24 h, monolayers were irradiated with an 658 nm laser operated at a power output of 11 mW in the continuos wave mode at different energy fluences of 1.86 J/cm2,3.72 J/cm2. Untreated cells were used as controls.The proliferation,ALP activity and the secretion of fibronectin of the laser and control groups were determined at different hours after irradiation. Results: The hPDLCs were cultured successfully.The MTT tests showed that laser irradiation for 1.86 J/cm2 and 3.72 J/cm2 significantly stimulated proliferation activity than the controls.hPDLCs exposed to 3.72 J/cm2 showed a significant increase in ALP activity.After 72 h of laser irridiation, energy density for 1.86 J/cm2 accelerated the synthesis of fibronectin in hPDLCs. Conclusion: Low-level 658 nm laser irridiation can promote the proliferation of hPDLCs.Suitable low-level laser irridiation is able to facilitate ALP activity and synthesis of fibronectin in hPDLCs.