组织荧光光谱技术可用来定量测量组织生物化学性质及组织结构的变化,进而实现疾病早期诊断.为提高组织荧光光谱检测的准确性,提出了组织荧光光谱检测系统校准流程及具体实现方法,包括激发光强校准、传输光路传光效率校准、探测模块波长校准和非线性校准、系统光谱响应校准、暗背景扣除、光谱平滑.搭建三套组织荧光光谱检测系统,并分别对荧光标准板和人体皮肤组织进行实验测试,结果显示未校准时三套系统对同一荧光板和同一受试者的荧光强度测量结果变异系数分别为7.4%和10.4%,校准后变异系数分别为0.9%和2.0%.

Fluorescence spectroscopy provides a noninvasive method to quantify biochemical and structural changes in tissues and it is regarded as an early subtle indicator of disease or injury.One challenge facing the rapid growth of fluorescence application is the inability to insure the accuracy of fluorescence measurements.The calibration protocols that include excitation energy calibration,collection efficiency calibration,wavelength calibration,system nonlinearity calibration,system response calibration,background subtraction and spectrum signal filtering,for optical instruments that ensure measurement accuracy are proposed.Three fluorescence spectroscopy systems are built and the fluorescence spectra of fluorescence standard and human skin are collected.The result shows that the spectrum intensity variation coefficient among these systems for the same reference standard is 7.4% and for the same human skin is 10.4% before calibration.By comparison,the spectrum intensity variation coefficients after calibration are 0.9% and 2.0%,respectively.