激光与光电子学进展, 2015, 52 (10): 101102, 网络出版: 2015-10-08
一种基于列文伯格-马克夸特的高精度荧光寿命计算成像方法
A High Precision Method for Analysis of Fluorescence Lifetime Imaging Microscopy Data Based on Levenberg-Marquardt Technique
成像系统 列文伯格-马克夸特 非线性最小二乘优化方法 门控荧光寿命成像系统 荧光寿命图像 imaging systems Levenberg-Marquardt nonlinear least squares optimization time-gated fluorescence lifetime imaging microscop fluorescence lifetime map
摘要
介绍了一种用于荧光寿命图像数据分析的高精度列文伯格-马克夸特(LM)迭代算法。该算法的性能经过标准荧光寿命试剂以及生物图像的算法验证。该算法适用于不同的荧光衰减模型，相对于普通的非线性最小二乘估计方法具有更高的精度。结果表明，列文伯格-马克夸特算法是一种高精度、适用性广的荧光寿命图像计算方法，可以满足生物学、生物化学、生物物理学、医学诊断等实际应用的需求。
Abstract
A high precision Levenberg-Marquardt (LM) iterative algorithm for the analysis of fluorescence lifetime imaging microscopy (FLIM) data is reported. The performance of this method was tested on the time-resolved fluorescence intensity images of fluorescence lifetime standard dyes and real biological images. The method is applicable for various fluorescence decay functions and has better estimation precision based on iterative nonlinear least squares minimization algorithm. It indicates that the LM iterative technique for FLIM analysis represents a precise and versatile method that enables practical applications of FLIM in biology, biochemistry, biophysics, and medical diagnosis.
刘洋, 周燕, 刘育梁. 一种基于列文伯格-马克夸特的高精度荧光寿命计算成像方法[J]. 激光与光电子学进展, 2015, 52(10): 101102. Liu Yang, Zhou Yan, Liu Yuliang. A High Precision Method for Analysis of Fluorescence Lifetime Imaging Microscopy Data Based on Levenberg-Marquardt Technique[J]. Laser & Optoelectronics Progress, 2015, 52(10): 101102.
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