从羊驼皮肤cDNA文库中筛选到RPL34的克隆, 经PCR鉴定并测序得知: 一种克隆的大小为270 bp（命名为RPL34 I）, 另一种克隆的大小为470 bp（命名为RPL34 II）。序列分析发现, RPL34 I和II在核苷酸水平上, 5’UTR区不具有同源性, 3’UTR区具有同源性; 在氨基酸水平上, 部分具有高度同源性; 分子进化树分析表明二者具有不同的进化速率。此外, RPL34 I和II对应于不同的转录本。通过以上结果分析, 认为RPL34 I和RPL34 II二者同时在羊驼皮肤中表达, 可能是RPL34的两个剪接体, 发挥不同的生物学功能。

RPL34 positive clones were screened from the cDNA library of alpaca skin. The sizes of the inserted fragments of clones were tested by PCR and sequenced. The results showed that the sizes of the inserted fragments were 270 bp (named as RPL34 I) and 470 bp (named as RPL34 II). Both of them were analyzed and found that they had homolog in 3’ UTR, but no homolog in 5’UTR on the nuclear acid lever, part of them had homolog in the amino acid encoded, they had different evolution rate, and they corresponded different transcripts. It was concluded by all those evidence that RPL34 I and RPL34 II were the possible splicing variants of RPL34gene. While being expressed in the alpaca skin at the same time, RPL34 I and RPL34 II may play different biological roles.