以甘薯( Ipomoea batatas (L.) Lam.) 品种栗子香的胚性悬浮细胞为受体材料, 用根癌农杆菌介导法, 获得了表达除草剂抗性基因bar基因的转HS1基因甘薯植株。共计380个遗传转化的胚性细胞团, 在添加2 mg/L 2,4-D、100 mg/L Carb 和10 mg/L Glu (glufosinate) 的固体MS 培养基上选择培养9周后, 得到了12个Glu抗性愈伤组织。将这些抗性愈伤组织转移到添加1 mg/L ABA、100 mg/L羧苄青霉素 和10 mg/L Glu的固体MS 培养基上, 其中的3个抗性愈伤组织再生出拟转基因植株。PCR鉴定它们为转基因植株。Southern blot 分析表明, HS1基因已整合到基因组中。转基因植株具有稳定的除草剂抗性。结薯观察实验结果表明, 转基因植株结薯正常。

Generation of transgenic sweet potato plants with HS1 expressing bar gene for herbicide resistance was achieved using Agrobacterium-mediated transformation and sweet potato (Ipomoea batatas (L.) Lam.) cv. Lizixiang embryogenic suspension cultures. Total 380 embryogenic cell aggregates were cocultivated with A. tumefaciens. Nine weeks after selection on MS medium supplemented with 2.0 mg/L 2,4-D, 100 mg/L Carb and 10 mg/L Glu(glufosinate), 12 Glu -resistant embryogenic calluses were produced. After transferred to MS medium supplemented with 1.0 mg/L ABA, 100 mg/L Carb and 10 mg/L Glu, 3 of them formed putative transgenic plants. PCR analysis indicated that 3 lines were transgenic. Stable integration of the bar gene into the genome of transgenic plants was confirmed by Southern blot analysis. Experiment result showed that transgenic plants exhibited functional expression of the bar gene by in vivo assay for herbicide resistance. Transgenic plants display complete roots.