激光生物学报, 2011, 20 (1): 22, 网络出版: 2015-10-08
60Co γ射线辐照和EMS处理对火龙果成活率及幼苗生长的影响
Effects of 60Co γ Irradiation and EMS Treatment on Survival Rate and Seeding Growth of Pitaya
火龙果 60Co γ射线 半致死剂量 EMS处理 半致死浓度 pitaya 60Co γ irradiation median lethal dose (LD50) EMS treatment median lethal concentration
摘要
采用不同剂量的60Co γ射线辐照火龙果枝条, 结果表明, 枝条的成活率随辐照剂量的增加而降低, 白肉火龙果枝条的适宜辐照范围为5 446.6~6 060.1 Rad, 红肉火龙果枝条的适宜辐照范围为4 444.1~4 981.7 Rad, 粉红火龙果枝条的适宜辐照范围为5 069.1~5 601.0 Rad; 辐照处理抑制了火龙果幼苗的生长, 且辐照剂量越大, 对火龙果幼苗的生长抑制作用越明显。采用不同浓度的EMS溶液处理火龙果嫩芽, 结果发现, 芽的成活率随EMS浓度增加和处理时间延长而逐渐减小, 适宜EMS诱变的浓度范围为0.34~0.41 mol/L; 随EMS浓度增加和处理时间的延长, 火龙果幼苗的高生长也明显受到抑制。
Abstract
The cutting branches of pitaya (Hylocereus SPP.) were irradiated by different dose 60Co γ-rays. The results indicated that survival rate of cutting branches decreased with the increase of irradiation dose, and the suitable irradiation dose rang for mutation breeding was 5 446.6~6 060.1 Rad of white flesh pitaya,4 444.1~4 981.7 Rad of red flesh pitaya, 5 069.1~5 601.0 Rad of pink flesh pitaya. Irradiation could inhibit the growth of young seedlings, and for the higher irradiation dose, the inhibition was more significant. The shoots of pitaya were treated with different EMS concentration. The results showed that survival rate of shoot decreased with EMS concentration increased and treatment time prolonged. The suitable EMS concentration rang for mutation breeding was 0.34~0.41 mol/L, and the higher EMS concentration and longer treatment time had obvious inhibition for height growth of pitaya seedlings.
邓仁菊, 范建新, 蔡永强, 高安辉, 袁启凤. 60Co γ射线辐照和EMS处理对火龙果成活率及幼苗生长的影响[J]. 激光生物学报, 2011, 20(1): 22. DENG Ren-ju, FAN Jian-xin, CAI Yong-qiang, GAO An-hui, YUAN Qi-feng. Effects of 60Co γ Irradiation and EMS Treatment on Survival Rate and Seeding Growth of Pitaya[J]. Acta Laser Biology Sinica, 2011, 20(1): 22.