激光生物学报, 2011, 20 (1): 103, 网络出版: 2015-10-08
拟南芥赤霉素受体AtGID1a原核表达条件的优化研究
Optimization for Prokaryotic Expression Conditions of Gibberellins Receptor AtGID1a in Arabidopsis
均匀设计 偏最小二乘回归 优化表达 pGEX-AtGID1a pGEX-AtGID1a uniform design partial least-squares regressive optimization expression
摘要
运用均匀设计法, 对影响Rossetta(DE3)/pGEX-KG/AtGID1a表达水平的因素进行了优化, 摸索出了对可溶性蛋白进行诱导表达的最优条件, 即蛋白胨16 g/L, 酵母粉5 g/L, 葡萄糖4 g/L, IPTG终浓度0.1 mmol/L, 诱导温度23 ℃, IPTG诱导时间4 h。在此条件下, 目的蛋白的平均密度从0.042提高到0.064, 优化率为52 %。
Abstract
The factors that influenced expression level of Rossetta(DE3)/pGEX-KG/AtGID1a were optimized using uniform design. The optimization induction condition for the solubility protein expression were investigated. The results were as followed: 16 g/L peptone, 5 g/L yeast powder, 4 g/L glucose, 0.1 mmol/L IPTG, 23 ℃ induction temperature and 4 h induction time. The average density of interest protein had increased from 0.042 to 0.064. The optimization rate was 52 %.
周敏, 赵卓亚, 徐文忠, 肖浪涛, 王若仲. 拟南芥赤霉素受体AtGID1a原核表达条件的优化研究[J]. 激光生物学报, 2011, 20(1): 103. ZHOU Min, ZHAO Zhuo-ya, XU Wen-zhong, ＸIAO Lang-tao, WANG Ruo-zhong. Optimization for Prokaryotic Expression Conditions of Gibberellins Receptor AtGID1a in Arabidopsis[J]. Acta Laser Biology Sinica, 2011, 20(1): 103.
PDF全文
