目的: 探讨阿魏酸对阿尔茨海默病(AD)模型小鼠神经行为学和海马胶质纤维酸性蛋白(GFAP)表达的影响, 分析阿魏酸对小鼠脑的保护作用。方法: 海马CA1区注射微量红藻氨酸(KA)建立痴呆模型, 然后对痴呆小鼠用不同剂量的阿魏酸(FA)灌胃治疗。Morris水迷宫实验观察小鼠行为学变化, 免疫组织化学方法观察GFAP的表达。结果: 与假手术组相比, 模型组学习记忆能力明显降低(P<0.01), GFAP阳性细胞表达明显增多(P<0.01); 与模型组相比, 阿魏酸治疗组学习记忆能力均明显提高(P<0.01), GFAP阳性细胞表达均明显减少(P<0.01)。结论: 用不同剂量的阿魏酸治疗拟AD小鼠后, 小鼠学习记忆能力得到明显改善, GFAP表达得到明显抑制, 起到保护脑的作用。

Objective: To investigate the effects of ferulic acid on neurological behavior and the expression of glial fibrillary acidic protein(GFAP) of hippocampus tissues in Alzheimer’s disease-like model mice, and to analyze the protective effects of ferulic acid on the brain. Methods: kainic acid(KA)was injected into hippocampus CAl region of mice and to establish Alzheimer’s disease-like model, then the drug group with different doses of ferulic acid gavage lasted a month. The learning and memory abilities of the mice were assessed through Morris water maze behavioral test, and GFAP were observed by immunohistochemistry respectively. Results: Compared with the normal group, the abilities of learning and memory of the mice in the model group significantly decreased(P<0.01) and the expression of GFAP in the CAl region were increased(P<0.01). Compared with the model group, the abilities of learning and memory of the mice in the ferulic acid group improved(P<0.01) and the expressions of GFAP in the CAl region decreased(P<0.01). Conclusion: The different doses of ferulic acid dealing with the Alzheimer disease-like model mice can improve the abilities of learning and memory of the mice and reduce the level of GFAP in the brain tissues of the mice with KA injury, which has significant protective effects on the brain．