以405只南江黄羊为试验动物, 以生长激素(GH)为候选基因, 利用PCR-SSCP技术和DNA序列分析技术筛选其多态位点, 并与南江黄羊的生长性状进行关联分析, 以期筛选与南江黄羊生长性状相关的分子标记。序列分析比对结果发现GH基因外显子4有个4 SNPs (27 G/A、68 C/A、136 G/A、170 C/T), 其中有3个SNPs引起了氨基酸替代。与南江黄羊生长性状的关联分析结果显示: 外显子4多态与初生重、2月龄体重、6月龄体重和部分体尺指标显著相关(P<0.05); FF基因型是初生重、2月龄体重、6月龄体重、2月龄体长和2月龄胸围的有利基因型。GH基因外显子4的多态可能是与南江黄羊生长性状相关的分子标记, 但还需要在南江黄羊群体中进一步的验证。

The aim of present study was to find the genetic markers associated with the growth traits in Nanjiang Huang goats for marker assisted selection line with higher growth rate. A total of 405 Nanjiang Huang goats were used to detect the polymorphisms of GH gene to analyze whether they could be candidate genes or molecular markers to improve the growth traits in Nanjiang Huang goats. The results of sequence analysis and megalign showed that 4 SNPs (27 G/A; 68 C/A; 136 G/A; 170 C/T) in exon 4 of GH gene. The correlations were observed between the polymorphisms of exon 4 and birth weight, the two-month body weight, the six-month body weight.Individuals with genotype FF had higher birth weight, two-month body weight and six-month body weight (P＜0.05). Polymorphisms of exon4 of GH gene may be important candidate gene controlling growth performance in Nanjiang Huang goats.