大气与环境光学学报, 2015, 10 (5): 376, 网络出版: 2015-10-22
基于24析因设计的重金属胁迫蛋白核小球藻联合毒性研究
Joint Toxicity of Multi-Heavy-Metal to Chlorella Pyrenoidosa Based on 24 Factorial Design
蛋白核小球藻 析因设计 重金属联合毒性 多元线性回归 chlorella pyrenoidosa factorial design joint toxicity of heavy metals multiple linear regression
摘要
设计实验研究多种重金属胁迫藻类的联合毒性,更有利于阐明真实环境中重金属与藻类的毒性作用过程和机理。 采用叶绿素荧光分析技术结合24析因设计方法研究重金属Hg2 + 、Cd2 + 、Cu2 + 和Zn2 + 胁迫蛋白核小球藻的联 合毒性作用,得到主效应Hg、Cd、Cu、Zn以及交互作用Hg*Cu、Hg*Zn和Hg*Cu*Zn对蛋白核小球藻潜在最大 量子效率Fv/Fm具有显著性影响(Sig.<0.05)的结论:通过多元线性回归拟合得到联合重金属浓度与藻 类光合活性抑制率间的剂量-效应关系,得到模型表达式: y[%]=-22.557+19.926Hg+5.282Cu+0.015Zn+0.029Hg*Zn-0.002Hg*Cu*Zn,并对 联合重金属胁迫蛋白核小球藻的机理做出解释;通过对模型进行统计 学检验和实验验证,证明该模型可用于实验室条件下已知种类重金属Hg2 + 、Cd2 + 、 Cu2 + 、Zn2 + 胁迫蛋白核小球藻的联合毒性估计。
Abstract
Designing experiments to research joint toxicity of multi-heavy-metal to algal is beneficial to illuminate toxic process and mechanism of heavy metals stress algal in real environment. Chlorophyll fluorescence analysis technique and 24 factorial design method were used to design and analyze joint toxicity of heavy metal Hg2 + , Cd2 + , Cu2 + and Zn2 + to chlorella pyrenoidosa. Results show that the main effects of Hg, Cd, Cu, Zn and interaction effects of Hg* Cu, Hg* Zn and Hg* Cu* Zn influence chlorella pyrenoidosa photosynthetic fluorescence parameters Fv/Fm significantly (Sig.<0.05). The relationship between the concentration of heavy metals and photosynthesis inhibition rate was established by using multiple linear regression method. The model is as follows: y[%]=-22.557+19.926Hg+5.282Cu+0.015Zn+0.029Hg*Zn- 0.002Hg*Cu*Zn, and it can be used to account for mechanism of heavy metals stressed chlorella pyrenoidosa. To test and verify the model by statistical method and experiment, the results show that the model can be used for estimating the joint toxicity of Hg2 + , Cd2 + , Cu2 + and Zn2 + stress chlorella pyrenoidosa in laboratory conditions.
段静波, 张玉钧, 赵南京, 殷高方, 肖雪, 方丽, 刘文清. 基于24析因设计的重金属胁迫蛋白核小球藻联合毒性研究[J]. 大气与环境光学学报, 2015, 10(5): 376. DUAN Jingbo, ZHANG Yujun, ZHAO Nanjing, YIN Gaofang, XIAO Xue, FANG Li, LIU Wenqing. Joint Toxicity of Multi-Heavy-Metal to Chlorella Pyrenoidosa Based on 24 Factorial Design[J]. Journal of Atmospheric and Environmental Optics, 2015, 10(5): 376.