激光生物学报, 2015, 24 (4): 364, 网络出版: 2015-11-30
NTERA-2的精原干细胞生物学特征鉴定
NTERA-2 cells with Characteristics of Spermatogonial Stem Cells
摘要
本研究通过免疫荧光分析，鉴定NTERA-2细胞是否具有精原干细胞特征。研究结果显示，重要精原干细胞标记分子GFRα-1和DDX4在NTERA-2细胞中均表现为阳性表达， NTERA-2细胞具有精原干细胞的特征。初步探讨了重要细胞信号转换蛋白JNK特异性抑制剂SP600125对NTERA-2细胞生长和增殖的影响，为进一步以NTERA-2细胞为精原干细胞模型开展JNK对生殖细胞发育调节功能的研究奠定基础。
Abstract
This study was to identify the characteristics of NTERA-2 cells to find whether out has it the characteristics of spermatogonial biology. Our results showed that NTERA-2 cells expressed some markers of spermatogonial stem cells, such as GFRα-1 and DDX4. It suggest that NTERA-2 cells maybe useful as a model for studying spermatogonial stem cell proliferation and differentiation in vitro. With the treatment by SP600125, the proliferation of NTERA - 2 cells is seriously inhibited. The results are the foundation of further research on the function of JNK in germ cell development.
王姿力, 周勇华, 罗超, 莫艳秀, 肖亚梅. NTERA-2的精原干细胞生物学特征鉴定[J]. 激光生物学报, 2015, 24(4): 364. WANG Zili, ZHOU Yonghua, LUO Chao, MO Yanxiu, XIAO Yamei. NTERA-2 cells with Characteristics of Spermatogonial Stem Cells[J]. Acta Laser Biology Sinica, 2015, 24(4): 364.
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