转移条件培养基是开展细胞间信号传递研究的常用实验手段。在开展辐射诱导旁效应的研究过程中,我们发现未照射的条件培养基也能够引起细胞DNA损伤,为探索DNA损伤的来源,进行了一系列实验。通过测定细胞活力水平、DNA损伤水平、细胞活性氧水平的变化,发现细胞在接受条件培养基之后,细胞活性氧水平上升,细胞活力下降,细胞内的DNA损伤水平上升。这些结果表明,相对于完全未处理的细胞,源于未进行辐照处理细胞的条件培养基能够影响细胞内的活性氧水平,进而引起受体细胞的DNA损伤。因此,在采用条件培养基转移方法的研究,尤其是涉及活性氧及DNA损伤的研究之中,必须要考虑作为对照的条件培养基对细胞的影响。

Conditioned medium transfer is a commonly used experimental means to carry out research about intercellular signal transmission. In the process of research on radiation induced bystander effects,it was found that conditioned medium harvested from unirradiated cells could induce DNA damage in recipient cells. The aim of this study is to investigate the source of DNA damage. Through evaluating the levels of reactive oxygen species and DNA damage of cells and the cell viability,it was found that after treated with conditioned medium,the levels of reactive oxygen species of cells were up-regulated,the cell viability was reduced and the DNA damage level was raised. These results suggested that compare to completely control cells,conditioned medium could influence the levels of reactive oxygen species of recipient cells. Therefore,the effect of conditioned medium to cells must be considered seriously in the studies using conditioned medium transfer,especially those involved reactive oxygen species and DNA damage.