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In vivo imaging of mice auricle vessels using adaptive optical confocal fluorescence microscope

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Abstract

Facilitated with stochastic parallel gradient descent (SPGD) algorithm for wavefront sensorless correcting aberrations, an adaptive optics (AO) confocal fluorescence microscopy is developed and used to record fluorescent signals in vivo. Vessels of mice auricle at 80, 100 and 120 μm depth are obtained, and image contrast and fluorescence intensity are significantly improved with AO correction. The typical 10%–90% rise-time of the metric value measured is 5.0 s for a measured close-loop bandwidth of 0.2 Hz. Therefore, the AO confocal microscopy implemented with SPGD algorithm for robust AO corrections will be a powerful tool for study of vascular dynamics in future.

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DOI:10.3788/col201513.111702

所属栏目:Medical optics and biotechnology

收稿日期:2015-08-15

录用日期:2015-09-29

网络出版日期:2015-10-21

作者单位    点击查看

Yi He:The Key Laboratory on Adaptive Optics, Chinese Academy of Sciences, Chengdu 61 0209, ChinaThe Laboratory on Adaptive Optics, Institute of Optics and Electronics, Chinese Academy of Sciences, Chengdu 6102 09, China
Zhibin Wang:The Key Laboratory on Adaptive Optics, Chinese Academy of Sciences, Chengdu 61 0209, ChinaThe Laboratory on Adaptive Optics, Institute of Optics and Electronics, Chinese Academy of Sciences, Chengdu 6102 09, ChinaUniversity of Chinese Academy of Sciences, Beijing 100039, China
Yuanyuan Wang:School of Optometry and Ophthalmology and Eye Hospital, Wenzhou Medical College, Wenzhou 3 2503 5, ChinaUniversity of Chinese Academy of Sciences, Beijing 100039, China
Ling Wei:The Key Laboratory on Adaptive Optics, Chinese Academy of Sciences, Chengdu 61 0209, ChinaThe Laboratory on Adaptive Optics, Institute of Optics and Electronics, Chinese Academy of Sciences, Chengdu 6102 09, China
Xiqi Li:The Key Laboratory on Adaptive Optics, Chinese Academy of Sciences, Chengdu 61 0209, ChinaThe Laboratory on Adaptive Optics, Institute of Optics and Electronics, Chinese Academy of Sciences, Chengdu 6102 09, China
Jinsheng Yang:The Key Laboratory on Adaptive Optics, Chinese Academy of Sciences, Chengdu 61 0209, ChinaThe Laboratory on Adaptive Optics, Institute of Optics and Electronics, Chinese Academy of Sciences, Chengdu 6102 09, China
Guohua Shi:The Key Laboratory on Adaptive Optics, Chinese Academy of Sciences, Chengdu 61 0209, ChinaThe Laboratory on Adaptive Optics, Institute of Optics and Electronics, Chinese Academy of Sciences, Chengdu 6102 09, China
and Yudong Zhang:The Key Laboratory on Adaptive Optics, Chinese Academy of Sciences, Chengdu 61 0209, ChinaThe Laboratory on Adaptive Optics, Institute of Optics and Electronics, Chinese Academy of Sciences, Chengdu 6102 09, China

联系人作者:联系作者(heyi_job@126.com)

备注:This work was supported by the Outstanding Young Scientists of the Chinese Academy of Sciences, the National Instrumentation Program (No.?2012YQ120080), the Zhejiang Province Technology Program (No.?2013C33170), the National Science Foundation of China (No.?61108082), the Sichuan Youth Science & Technology Foundation (No.?2013JQ0028), and the West Light Foundation of the Chinese Academy of Sciences. The authors would like to thank Hao Li and Jing Lu for many helpful discussions regarding this project.

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引用该论文

Yi He, Zhibin Wang, Yuanyuan Wang, Ling Wei, Xiqi Li, Jinsheng Yang, Guohua Shi, and Yudong Zhang, "In vivo imaging of mice auricle vessels using adaptive optical confocal fluorescence microscope," Chinese Optics Letters 13(11), 111702 (2015)

被引情况

【1】Wenjin Liu,Lizhi Dong,Ping Yang,and Bing Xu. Zonal decoupling algorithm for dual deformable mirror adaptive optics system. Chinese Optics Letters, 2016, 14(2): 20101-20105

【2】Shuheng Chi,Liang Li,and Yiqun Wu. Preparation of two-photon fluorescent probe and biological imaging application in cells. Chinese Optics Letters, 2016, 14(6): 61603-61607

【3】Lin Zhang,Chuangjian Cai,Yanlu Lv,and Jianwen Luo. Early-photon guided reconstruction method for time-domain fluorescence lifetime tomography. Chinese Optics Letters, 2016, 14(7): 71702-71706

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