Chinese Optics Letters, 2015, 13 (11): 111702, Published Online: Sep. 13, 2018   

In vivo imaging of mice auricle vessels using adaptive optical confocal fluorescence microscope

Author Affiliations
1 The Key Laboratory on Adaptive Optics, Chinese Academy of Sciences, Chengdu 610209, China
2 The Laboratory on Adaptive Optics, Institute of Optics and Electronics, Chinese Academy of Sciences, Chengdu 610209, China
3 School of Optometry and Ophthalmology and Eye Hospital, Wenzhou Medical College, Wenzhou 325035, China
4 University of Chinese Academy of Sciences, Beijing 100039, China
Abstract
Facilitated with stochastic parallel gradient descent (SPGD) algorithm for wavefront sensorless correcting aberrations, an adaptive optics (AO) confocal fluorescence microscopy is developed and used to record fluorescent signals in vivo. Vessels of mice auricle at 80, 100 and 120 μm depth are obtained, and image contrast and fluorescence intensity are significantly improved with AO correction. The typical 10%–90% rise-time of the metric value measured is 5.0 s for a measured close-loop bandwidth of 0.2 Hz. Therefore, the AO confocal microscopy implemented with SPGD algorithm for robust AO corrections will be a powerful tool for study of vascular dynamics in future.

Yi He, Zhibin Wang, Yuanyuan Wang, Ling Wei, Xiqi Li, Jinsheng Yang, Guohua Shi, Yudong Zhang. In vivo imaging of mice auricle vessels using adaptive optical confocal fluorescence microscope[J]. Chinese Optics Letters, 2015, 13(11): 111702.

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