Chinese Optics Letters, 2015, 13 (11): 111702, Published Online: Sep. 13, 2018
In vivo imaging of mice auricle vessels using adaptive optical confocal fluorescence microscope
110.0110 Imaging systems 170.0170 Medical optics and biotechnology 170.1790 Confocal microscopy 170.0180 Microscopy
Abstract
Facilitated with stochastic parallel gradient descent (SPGD) algorithm for wavefront sensorless correcting aberrations, an adaptive optics (AO) confocal fluorescence microscopy is developed and used to record fluorescent signals in vivo . Vessels of mice auricle at 80, 100 and 120 μm depth are obtained, and image contrast and fluorescence intensity are significantly improved with AO correction. The typical 10%–90% rise-time of the metric value measured is 5.0 s for a measured close-loop bandwidth of 0.2 Hz. Therefore, the AO confocal microscopy implemented with SPGD algorithm for robust AO corrections will be a powerful tool for study of vascular dynamics in future.
Yi He, Zhibin Wang, Yuanyuan Wang, Ling Wei, Xiqi Li, Jinsheng Yang, Guohua Shi, Yudong Zhang. In vivo imaging of mice auricle vessels using adaptive optical confocal fluorescence microscope[J]. Chinese Optics Letters, 2015, 13(11): 111702.