中国激光, 2016, 43 (1): 0115003, 网络出版: 2015-12-21
拉曼光谱技术测量单个红细胞的血红蛋白浓度
Measurement of Hemoglobin Concentration of Single Red Blood Cell Using Raman Spectroscopy
光谱学 拉曼光谱 血红蛋白 单个红细胞 平均红细胞血红蛋白浓度 spectroscopy Raman spectroscopy hemoglobin single red blood cell mean corpuscular hemoglobin concentration
摘要
单细胞光镊拉曼光谱技术测量单个红细胞的血红蛋白浓度,配制不同浓度的血红蛋白溶液后利用拉曼光谱强度与血红蛋白浓度的关系,测量单个红细胞的拉曼光谱,根据公式计算单个红细胞内血红蛋白浓度。测量了70个血红细胞的拉曼光谱,结果显示单个红细胞的血红蛋白浓度为270~500 g/L,其中血红蛋白浓度在350~450 g/L 之间的是成熟的红细胞,占红细胞总数的90%以上,而浓度在300 g/L 以下的可能是网织红细胞,浓度在450 g/L 以上的是老化了的红细胞。这与平均红细胞血红蛋白浓度(MCHC)310~370 g/L 基本一致。表明利用该方法可以对单个红细胞内的血红蛋白浓度进行快速、无损、简单、精确的定量分析,展现了拉曼光谱技术在单细胞分析上的优势。
Abstract
Hemoglobin concentrations of single red blood cells are measured by single cell laser tweezers Raman spectroscopy. Hemoglobin solution with different concentrations is prepared and the linear relationship between Raman intensity and hemoglobin concentration is obtained. By detecting the Raman spectrum of a single red blood cell, the hemoglobin concentration of a single red blood cell is calculated according to the linear formula. Raman spectra of 70 red blood cells are measured. The calculated hemoglobin concentration of single red blood cells is from 270 g/L to 500 g/L. Mature erythrocytes account for more than 90% of the red blood cells and its hemoglobin concentration is 350~450 g/L. The hemoglobin concentration of reticulocyte is 300 g/L or less, and the hemoglobin concentration of old red blood cells is higher than 450 g/L. The conclusion conforms to the standard of mean corpuscular hemoglobin concentration (MCHC) 310~370 g/L. Using this method to measure hemoglobin concentration of single red blood cells is quick, non-destructive, simple and accurate. This shows the advantages of Raman spectroscopy on single cell analysis.
徐斌, 林漫漫, 姚辉璐, 刘军贤. 拉曼光谱技术测量单个红细胞的血红蛋白浓度[J]. 中国激光, 2016, 43(1): 0115003. Xu Bin, Lin Manman, Yao Huilu, Liu Junxian. Measurement of Hemoglobin Concentration of Single Red Blood Cell Using Raman Spectroscopy[J]. Chinese Journal of Lasers, 2016, 43(1): 0115003.