光学相干层析(OCT)是一种高分辨率、高灵敏度、无损伤的断层深度探测方法。搭建了一台手持式谱域光学相干层析(SDOCT)系统，对人体牙齿组织进行成像。介绍了手持式OCT探头设计。该手持式探头利用扫描振镜进行横向扫描，结构简单且十分紧凑，可以稳定地检测活体组织。利用该系统分别对离体牙齿和活体牙齿进行成像。通过实验结果可以清晰地看到活体牙齿的牙釉质、牙本质和釉质本质界面等组织，实现了高分辨率的断层成像。

Optical coherence tomography (OCT) is a non-invasive high resolution cross-sectional imaging method with high sensitivity. A simple handheld spectral- domain optical coherence tomography (SDOCT) system is developed for imaging tooth tissues. The design of the handheld OCT probe is described in detail, and the lateral scanning is realized by using a scanning mirror. The handheld OCT probe is characterized by its compact size and portable feature, making it easier for in vivo tooth imaging. The system is used for imaging of in vitro and in vivo tooth tissues. The enamel, dentin and the interface between them can be clearly observed in the high-resolution tomographic images.