作为一种常规光谱测量技术, 拉曼光谱仪应用广泛并具有特定的校准方法。 主要面向激光共聚焦显微拉曼光谱仪, 概括介绍了激光共聚焦显微拉曼光谱仪的测量原理、 结构及主要组成部分, 重点介绍了激光共聚焦显微拉曼光谱仪的校准项目和校准过程, 重点是分光计(摄谱仪)校准和激光器激发波长校准。 在传统校准方法的基础上提出了一种更准确、 新的获得实际激发波长的方法, 即在Δν=0处校准的方法。 根据此新的激发波长校准方法测量单质硫的拉曼频移, 并将硫的拉曼频移值与美国材料与试验协会(ASTM)值比较。 结果表明此校准步骤正确, 从而进一步提出了一种更为合理的激光共聚焦显微拉曼光谱仪的校准程序。

As a common spectral characterization technique, Raman spectroscopy is widely used and has a specified calibration procedure. Based on laser confocal micro-Raman spectrometer, in this paper, we briefly introduced the principle, configuration and main components of Raman spectrometer. In addition, the calibration procedures were also presented, with an emphasis on the calibration of spectrometer (spectrograph) and that of excitation laser wavelength. On the basis of conventional calibration method, a novel and more accurate method was proposed to obtain the actual excitation wavelength, that is, calibration at the point of Raman shift Δν=0. Using this novel calibration method of excitation wavelength, Raman frequency shift values of sulfur were measured, and compared with the standard values from American Society Testing and Materials (ASTM). As a result, the measured values after calibration were consistent with those ASTM values, which indicated that the calibration method is accurate. Thus, a more reasonable calibration procedure of the laser confocal micro-Raman spectrometer was provided.