利用傅里叶变换红外(FTIR)光谱技术对三种柳树正常叶和病叶, 同一枝条的幼叶、成叶、黄叶、病叶进行光谱测试, 旨在分析柳树病叶发生的一些生理变化, 并探讨柳树病变对系统聚类的影响。谱图显示不同阶段的叶片光谱变化主要在1800～800 cm-1。对此波段进行二阶导数处理, 清晰可看到蛋白质和木质素的叠加区域1700～1500 cm-1, 草酸钙特征峰1621、1318 cm-1, 糖类特征峰1075 cm-1附近峰强有明显变化, 用吸光度比A1621/A3390、A1318/A3390、A1075/A3390来比较草酸钙和糖类相对含量变化, 用1700～1500 cm-1波段的拟合峰面积比S酰胺I/S木质素、S酰胺Ⅱ/S木质素和S酰胺Ⅱ/S酰胺I来比较蛋白质相对含量变化。结果显示, 病叶的草酸钙相对含量增加、多糖和蛋白质相对含量在减少。将叶片1800～800 cm-1范围的二阶导数进行系统聚类, 发现病叶化学成分的改变没有影响聚类效果, 聚类正确率达100%。

Using Fourier transform infrared (FTIR) spectroscopy of three willow leaf and leaf disease, the young leaves of the same branch and leaves, yellow leaves, leaf disease spectrum testing, aims to analyze the willow leaf disease of some physiological changes, to explore the effect of disease leaves on the clustering of the system. Infrared spectroscopy blade at different stages of major change in 1800~800 cm-1, this bands second derivative processing, you can clearly see the superimposed region of the protein and lignin 1700~1500 cm-1, characteristic peaks of calcium oxalate 1621, 1318 cm-1, characteristic peaks carbohydrate peak near 1075 cm-1 strong there are significant changes, with absorbance ratio A1621/A3390, A1318/A3390, A1075/A3390 to compare the relative content of calcium oxalate and sugar, with 1700~1500 cm-1 band fitting peak area ratio Samide I /Slignin, SamideⅡ/Slignin and SamideⅡ/Samide I used to compare the relative amounts of the protein. The results showed that the relative contents of calcium oxalate in the diseased leaves were increased, and the relative contents of polysaccharide and protein decreased. The blade 1800~800 cm-1 range of the second derivative of systematic cluster found diseased leaves change did not affect the chemical composition of the clustering effect, clustering correct rate of 100%.