Chinese Optics Letters, 2016, 14 (6): 061603, Published Online: Aug. 6, 2018  

Preparation of two-photon fluorescent probe and biological imaging application in cells Download: 772次

Author Affiliations
1 Key Laboratory of Material Science and Technology for High Power Lasers, Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800, China
2 Key Lab of Functional Inorganic Material Chemistry (Heilongjiang University), Ministry of Education, Harbin 150080, China
Abstract
We report on a novel dibenzothiophene-based two-photon fluorescent probe for selective nuclear bioimaging, which contains bilaterally symmetrical pyridine rings connected by a central conjugated-bridge dibenzothiophene. This probe possesses a large two-photon absorption cross-section of 471 GM, yields a 25-fold enhancement of the fluorescence titration, and a stronger photostability for nuclei labeling than existing probes. The real-time observation period is a minimum of 1800 s under a femtosecond laser excitation, which is significantly longer than that of 4,6-diamidino-2-phenylindole. The above results confirm that this novel molecule is a suitable two-photon fluorescent probe for application to nuclear bioimaging in cells.

Shuheng Chi, Liang Li, Yiqun Wu. Preparation of two-photon fluorescent probe and biological imaging application in cells[J]. Chinese Optics Letters, 2016, 14(6): 061603.

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