Chinese Optics Letters, 2016, 14 (6): 061603, Published Online: Aug. 6, 2018
Preparation of two-photon fluorescent probe and biological imaging application in cells Download: 772次
160.0160 Materials 160.4670 Optical materials 160.2540 Fluorescent and luminescent materials 190.4400 Nonlinear optics, materials 180.2520 Fluorescence microscopy 170.3880 Medical and biological imaging
Abstract
We report on a novel dibenzothiophene-based two-photon fluorescent probe for selective nuclear bioimaging, which contains bilaterally symmetrical pyridine rings connected by a central conjugated-bridge dibenzothiophene. This probe possesses a large two-photon absorption cross-section of 471 GM, yields a 25-fold enhancement of the fluorescence titration, and a stronger photostability for nuclei labeling than existing probes. The real-time observation period is a minimum of 1800 s under a femtosecond laser excitation, which is significantly longer than that of 4 ′ ,6-diamidino-2-phenylindole. The above results confirm that this novel molecule is a suitable two-photon fluorescent probe for application to nuclear bioimaging in cells.
Shuheng Chi, Liang Li, Yiqun Wu. Preparation of two-photon fluorescent probe and biological imaging application in cells[J]. Chinese Optics Letters, 2016, 14(6): 061603.