采用532 nm、632.8 nm和785 nm三种不同波长激光束, 研制适用于各波长的TiO2-AgNPs基底, 检测罗丹明6g(R6g)和健康人血清两种样品的表面增强喇曼光谱(SERSp), 并验证不同波长激光激励对SERSp指纹谱的影响.结果表明：当提拉速度达到200 mm/min时, TiO2薄膜光催化活性不再增加；在440℃~600℃范围内, TiO2晶型均为锐钛矿结构；膜厚、温度及紫外灯照射时间共同影响银粒子生长, 200 mm/min提拉速度、520 ℃煅烧温度及紫外光照射80 min条件下获得的TiO2-AgNPs基底具有较好喇曼增强效果；不同波长激励对SERSp各谱线的增强影响明显, 在532 nm波长激励的SERSp中, 大波数的谱峰增强因子明显高于小波数的谱峰, 在785 nm波长激励的SERSp中正好相反, 而在632.8 nm波长激励的SERSp中, 大、小波数谱峰增强因子比较均一；不同波长激励样品SERSp指纹谱有明显的差异.

Three different lasers with the wavelengths of 532, 632.8 and 785 nm, were used and have been given a preferable TiO2-AgNPs basement suited to each wavelength. On this condition,Raman spectra experiment of R6G and serum were conducted. When the lifting speed is up to 200 mm/min, photocatalytic activity of TiO2 thin films is no longer increased. In the range of 440~600 degrees, Titania crystal is still an anatase structure. Film thickness, temperature and ultraviolet irradiation time have an influence on the growth of silver particles together, 200 mm/min pulling speed, 520 ℃ calcination temperature and UV irradiation 80 min's condition can give TiO2-AgNPs basement a better Raman enhancement factor.It is found that the excitation laser with different wavelength has an obvious effect on spectrum.For 532 nm ,the enhancement factor of large wave number'spectral peak is significantly higher than that of small wave number,the conclusion is just opposite when laser of 785nm is used.SERSp drived by 632.8nm of the laser wavelength is different form the other two consequences and show uniform enhancement factor between large and small wave number.Paper analyzes fluorescence excitation's contribution to the Raman enhancement may be the main influence factors which lead to the SERp's significant difference .