应用激光, 2016, 36 (2): 151, 网络出版: 2016-06-06
激光制备+电极化处理梯度生物陶瓷涂层的生物活性
Biological Activity of Gradient Ceramic Coating Prepared by Laser and Electric Polarization Treatment
宽带激光制备 电极化处理 表面形貌 生物活性 细胞定向分化 wide band laser preparation electric polarization treatment surface morphology biological activity cell differentiation
摘要
采用宽带激光熔覆技术, 梯度设计的思想和碱液环境中电极化后的处理方法来改善涂层表面形貌和提高涂层生物活性、细胞相容性, 制备了一种梯度生物活性陶瓷涂层。利用SEM分析手段对涂层表面形貌进行研究;通过模拟体液(SBF)浸泡实验考察涂层活性;采用人成骨细胞与涂层材料共培养的方式, 验证了细胞在涂层表面的增殖和定向分化能力。结果表明, 激光制备+电极化处理涂层表面形貌是典型的羟基磷灰石(HA)结构;激光制备+电极化处理涂层表面生成的HA+β-TCP相更多, 生物活性更好;激光制备+电极化处理涂层表面细胞的增殖和定向分化能力更好。
Abstract
A gradient bioactive ceramic coatings were prepared by the method of wide-band laser cladding technique in this paper, gradient design thought and electric polarization processing method alkaline environment in alkaline environment were to improve the surface morphology of the coating and improve the coating biological activity and cell compatibility. By using SEM analysis method the surface morphology of the coating were studied. Active coating were investigated by using simulated body fluid (SBF) immersion test; By co-culture of bone cells and coating material, the cells in the surface coating of proliferation and differentiation were verified. The results show that the laser system and electric polarization coating surface morphology is typical of hydroxyapatite (HA) structure. Laser preparation and electric polarization processing coating surface formed more HA+β-tcp, and has better biological activity. Laser preparation and electric polarization treatment of proliferation and differentiation of the cells on the surface of the coating are better.
田兴强, 汪震. 激光制备+电极化处理梯度生物陶瓷涂层的生物活性[J]. 应用激光, 2016, 36(2): 151. Tian Xingqiang, Wang Zhen. Biological Activity of Gradient Ceramic Coating Prepared by Laser and Electric Polarization Treatment[J]. APPLIED LASER, 2016, 36(2): 151.