目的：从微渗漏角度探究两种脉冲的Er:YAG激光联合自酸蚀粘接剂对于窝沟封闭的影响。方法：50颗离体牙随机分为5组, 每组10颗, 第1组：传统磷酸蚀刻处理窝沟釉质表面;第2组：MSP(最短脉冲)模式下的激光处理窝沟釉质表面;第3组：MSP+自酸蚀粘接系统处理窝沟釉质表面;第4组：SP(短脉冲)模式下的激光处理窝沟釉质表面;第5组：SP+自酸蚀粘接系统处理窝沟釉质表面。然后对所有组进行封闭剂充填。最后用体视显微镜进行微渗漏的观察。结果: 两种脉冲的激光比较, 差异无统计学意义(P＞0.05);对照组与激光组比较, 差异有统计学意义(P＜0.05);对照组与激光+自酸蚀组比较, 差异无统计学差异(P＞0.05)。结论：激光脉冲对窝沟封闭微渗漏无影响, 单独使用激光不能代替全酸的作用, 但是联合使用自酸蚀粘接系统能达到传统方法的效果, 有望应用于临床。

Objective: This study was to investigate the marginal microleakage in the fissure sealants that conditioned with two kinds of pulse Er:YAG laser combined with self-etching adhesives. Methods: In this study, 50 teeth were divided into 5 groups.(1)acid etching,(2)Er:YAG(MSP),(3)Er:YAG(MSP)+acid etching,(4)Er:YAG(SP),(5)Er:YAG(SP)+acid etching. All of the five groups were filled with fissure sealants. The microleakage tests were performed, observed and scored under stereomicroscope. Results: There was no difference between these two pulses groups(P>0.05). The laser group had higher microleakage than the acid etching group(P<0.05). There was no difference between the acid etching group and the Er:YAG laser+self-etching adhesives group. Conclusions:The pulses’ differences didn’t affect the amount of microleakage. Only combined with self-etching adhesives, the Er:YAG laser can make the same amount microleakage with the acid etching in the fissure sealants. There is possibility to apply this combination to the patients.