为了能对自主研制的脑肿瘤手术医用显微成像光谱仪进行光谱定标, 设计了由单色仪、钨灯光源、棱镜-光栅-棱镜成像光谱仪及手术显微平台组成的光谱定标系统。采用单色仪波长扫描法, 自主开发了相应的光谱定标系统软件, 获得了显微成像光谱仪全谱段的光谱数据, 完成了数据处理和分析等工作。通过调整光路、单色仪定标、成像光谱仪定标3个步骤实现了系统的光谱定标。定标结果表明: 显微成像光谱仪的光谱区大于400~900 nm; 定标精度高于0.1 nm, 光谱分辨率高于3 nm, 各项特征指标均高于设计指标。测试验证实验表明, 所建立的光谱定标系统定标精准,结构简单、紧凑,操作简单, 符合显微成像光谱仪的实际临床应用要求。

To accomplish the spectral calibration of a medical microscopic imaging spectrometer developed by ourselves for brain tumor operation, a spectral calibration system was designed. The system is composed of a monochromator, a tungsten-light source, a surgical microscope platform and a prism-grating-prism(PGP) imaging spectrometer. A software for spectral calibration was developed based on monochromator wavelength scanning method, the spectral data of the medical microscopic imaging spectrometer in full bands were obtained and the data processing and data analysis were performed. The spectral calibration was implemented by adjusting path, monochromator calibration and imager calibration. Experimental results show that the accuracy of the spectral calibration is better than 0.1 nm, the spectral ranges of the imaging spectrometer are greater than 400—900 nm and its spectral resolution is better than 3 nm. The test validation shows that the spectral calibration system is accurate, simple, compact, and easy to operate and meets the actual clinical requirements of microscopic imaging spectrometers.