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基于可变光脉冲诱导荧光的浮游植物光合作用参数测量系统设计

Design of Phytoplankton Photosynthetic Parameter Measurement System Based on Variable Pulse Induced Fluorescence

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摘要

浮游植物光合作用参数的快速测量对水华和赤潮灾害预警及水体生态研究具有重要作用。针对高速重复脉冲(FRR)光合作用参数测量技术中由窄脉冲光源激发引起的高数据采样率问题,采用单光脉冲实现单周转模式激发,应用脉冲积分法实现弛豫模式微弱光脉冲信号检测,设计了基于可变光脉冲诱导荧光的浮游植物光合作用参数测量系统,将数据采样率需求由10 MS/s以上降低至1 MS/s。实现了浮游植物光系统II(PSII)功能吸收截面、PSII最大光化学量子产率以及QA-(还原后的初级电子受体)再氧化时间常数的快速测量。实验表明,测量结果的相对标准偏差均小于3%。

Abstract

Rapid measurement of phytoplankton photosynthetic parameters is important for bloom and red tide prediction as well as water ecological research. In order to solve the problem of high data sampling rate caused by narrow pulse light source when the fast repetition rate (FRR) method is applied, a phytoplankton photosynthetic parameter measurement system based on variable pulse induced fluorescence is designed. The designed system utilizes a single pulse to realize the single turnover excitation mode, and employs pulse integration to implement weak light pulse signal detection in the relaxation mode. The data sampling rate can be reduced from higher than 10 MS/s to 1 MS/s. The functional absorption cross section of photosystem II (PSII), maximum quantum yield of photochemistry in PSII, and QA- (reduced primary electron acceptor) reoxidation time constant, can be measured rapidly. The relative standard deviation of the three parameters is less than 3%.

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中图分类号:O439

DOI:10.3788/lop53.072301

所属栏目:光学器件

基金项目:国家自然科学基金(31400317)、安徽省高校自然科学研究重点项(KJ2016A594,KJ2014A211)、安徽省自然科学基金(1408085MD72)

收稿日期:2016-02-01

修改稿日期:2016-03-04

网络出版日期:2016-06-22

作者单位    点击查看

石朝毅:合肥学院电子信息与电气工程系, 安徽 合肥 230601
高先和:合肥学院电子信息与电气工程系, 安徽 合肥 230601
殷高方:中国科学院安徽光学精密机械研究所国家环境保护环境光学监测技术重点实验室, 安徽 合肥 230031
周泽华:合肥学院电子信息与电气工程系, 安徽 合肥 230601
卢军:合肥学院电子信息与电气工程系, 安徽 合肥 230601
胡学友:合肥学院电子信息与电气工程系, 安徽 合肥 230601

联系人作者:石朝毅(chaoyi@mail.ustc.edu.cn)

备注:石朝毅(1986—), 男, 博士, 讲师, 主要从事光电信息处理方面的研究。

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引用该论文

Shi Chaoyi,Gao Xianhe,Yin Gaofang,Zhou Zehua,Lu Jun,Hu Xueyou. Design of Phytoplankton Photosynthetic Parameter Measurement System Based on Variable Pulse Induced Fluorescence[J]. Laser & Optoelectronics Progress, 2016, 53(7): 072301

石朝毅,高先和,殷高方,周泽华,卢军,胡学友. 基于可变光脉冲诱导荧光的浮游植物光合作用参数测量系统设计[J]. 激光与光电子学进展, 2016, 53(7): 072301

被引情况

【1】张小玲,殷高方,赵南京,杨瑞芳,覃志松,陈双,甘婷婷,肖雪,段静波,刘建国,刘文清. 基于加权平均法的活体藻类三维荧光标准光谱构建. 光学学报, 2018, 38(7): 730001--1

【2】王翔,殷高方,赵南京,甘婷婷,覃志松,陈敏,华卉,董鸣,丁志超,亓培龙,王璐,孟德硕,刘建国. 基于TPLIF技术的光合荧光参数快速准确测量的激发光强自适应方法. 光学学报, 2020, 40(24): 2412001--1

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