本文介绍浸泡法制备基于滤纸的SERS基底,并分析滤纸SERS基底表面银纳米粒子(AgNP)的分布与浸泡时间的关系。以精浆为检测对象,相比于514 nm波长激发，785 nm激发可获得更好的光谱数据，同时还比较了该波长激发下精浆的常规拉曼光谱与SERS光谱。更为重要的是,通过采用精浆中654 cm-1谱峰强度评估不同浸泡时间下(6 h,12 h,24 h)滤纸SERS基底的增强性能和测量结果的重复性。实验结果表明,12 h浸泡获得的滤纸SERS基底表面具有均匀的AgNP分布,在785 nm波长激发下,纸基SERS基底可提供增强效果及光谱重复性俱佳的精浆SERS光谱。

This article introduced a filter paper-based SERS substrate through soaking method,and investigated the relationship between the distributions of silver nanoparticles(AgNPs)on the filter paper with soaking time in detail.SERS spectra of seminal plasma under different excitation wavelengths(514 nm and 785 nm)were compared,and the latter was confirmed to demonstrate better SERS signals.SERS and normal Raman spectra of seminal plasma using 785 nm excitation laser was compared as well.More importantly,the intensity of the strongest peak(654 cm-1)from SERS spectra of seminal plasma was used to evaluated the enhancement effects and signal reproducibility with filter paper SERS substrates under different soaking time(6 h,12 h,24 h).Our results indicated that the AgNPs on the paper substrate soaked with concentrated AgNP colloid presented a relatively uniform distribution at the soaking time of 12 h and the substrate demonstrated high Raman signal enhancement and good signal reproducibility as well.