光电工程, 2016, 43 (8): 18, 网络出版: 2016-09-12  

介质膜结构空芯光纤酶基传感器的制作与优化

Fabrication and Optimization of Enzyme Biosensors Based on Hollow Fiber with Dielectric Film
作者单位
复旦大学通信科学与工程系,上海 200433
摘要
设计了一种基于介质膜结构的空芯光纤化学发光传感器。建立了多层膜空芯光纤非子午线几何光学传输模型,对其损耗谱和发光特性进行了仿真分析,并考虑了介质膜界面粗糙度、介质膜厚度等相关参数对化学发光及传输特性的影响。改进了溶胶 -凝胶酶基敏感膜的制作工艺,提出了分离镀膜法,并对敏感膜厚度进行了实测分析。搭建了基于介质膜空芯光纤化学发光传感器的检测系统,对过氧化氢浓度进行了实际测量。结果表明,基于本传感器的系统,得到了 22.8 nW/(mmol·L-1)的灵敏度以及 3 μmol/L的检测极限。实现了近 200 h的使用寿命。且操作简单,反应快速,可小型化,可应用于环境监测,生物分析等领域。
Abstract
A chemiluminescence sensor is proposed based on the dielectric coated silver hollow fiber. A ray optics model is established for the multi-layer hollow fiber to analyze the loss spectrum and chemiluminescent characteristics. And parameters such as film surface roughness and thickness are taken into consideration. The fabrication techniques for coating sol-gel sensing film inside the hollow fiber are developed. A sensing system is designed and established for detecting hydrogen peroxide concentration and a high sensitivity (22.8 nW/(mmol·L-1)) and good detection limit (3μmol/L) are achieved. This sensor has a good performance in 200 hours. It has potential applications in environmental monitoring and biological analysis fields for its simple operation, fast response, and miniaturization.

何宇婧, 陈盼, 朱晓松, 汤晓黎, 石艺尉. 介质膜结构空芯光纤酶基传感器的制作与优化[J]. 光电工程, 2016, 43(8): 18. HE Yujing, CHEN Pan, ZHU Xiaosong, TANG Xiaoli, SHI Yiwei. Fabrication and Optimization of Enzyme Biosensors Based on Hollow Fiber with Dielectric Film[J]. Opto-Electronic Engineering, 2016, 43(8): 18.

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