光学学报, 2016, 36 (10): 1018001, 网络出版: 2016-10-12  

共焦布里渊显微镜轴向成像特性

Axial Imaging Property of Confocal Brillouin Microscopy
作者单位
北京理工大学精密光电测试仪器及技术北京市重点实验室, 北京 100081
摘要
搭建了共焦布里渊显微镜装置,采用532 nm的单模激光器激发样品布里渊散射光,显微物镜放大倍数为100倍,数值孔径为0.8,采用串联扫描式多通道法布里-珀罗(F-P)干涉仪收集布里渊光。从实验中测得了装置的共焦光强响应曲线,以及二氧化硅玻璃、硅胶和聚甲基丙烯酸甲酯(PMMA)3种样品的布里渊光谱。采用全新的光子数组分权值数据处理方法仿真分析了此装置的轴向成像分辨力,以及测试样品为二氧化硅玻璃-硅胶-PMMA多层样品时,样品的频移、轴向声速和纵向弹性模量3种参量的轴向成像特性,并对此方法获得的3种参量的轴向成像特性曲线进行了误差分析。仿真结果显示,光子数组分权值数据处理方法可使装置的轴向成像分辨力提高至约2 μm。在信噪比高于1.46 dB时,通过误差关系曲线可获得精确的布里渊频移值、轴向声速和纵向弹性模量。
Abstract
Confocal Brillouin microscopy device is set up, and Brillouin scattering light of sample is excited using a single-mode laser of 532 nm wavelength. The amplification factor is 100× and the numerical aperture is 0.8. A tandem scanning multi-channel Fabry-Perot (F-P) interferometer is used to collect Brillouin light. In the experiment, the confocal light intensity response curve is measured and the Brillouin spectra of SiO2 glass, silicon rubber, and polymethyl methacrylate (PMMA) are obtained. Using a novel photon number component factor data process method, the axial imaging resolution is simulated and analyzed. The axial imaging properties of frequency shift, axial acoustic velocity and longitudinal elastic moduli of multi-layer sample of SiO2 glass-silicon rubber-PMMA are also presented. Error analysis of these three parameters of axial imaging property curve by this method is made as well. The simulation results show that the axial imaging resolution can be improved to about 2 μm with this novel photon number component factor data process method. When the signal-to-noise ratio is greater than 1.46 dB, the accurate Brillouin frequency shift, axial acoustic velocity and longitudinal elastic moduli can be obtained by error relation curves.

钟昆, 邱丽荣, 吴寒旭, 赵维谦. 共焦布里渊显微镜轴向成像特性[J]. 光学学报, 2016, 36(10): 1018001. Zhong Kun, Qiu Lirong, Wu Hanxu, Zhao Weiqian. Axial Imaging Property of Confocal Brillouin Microscopy[J]. Acta Optica Sinica, 2016, 36(10): 1018001.

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