氧化亚铁钩端螺旋菌(Leptospirillum ferrooxidans, L.f)是一种极端嗜酸, 专性自养氧化铁的细菌, 能够耐受较低pH和较高的温度, 被广泛应用于生物浸矿和环境治理。氧化亚铁钩端螺旋体菌的生物浸矿效率与其对Fe2+氧化速率相关, 因此, 本文采用响应面法, 通过建立二次多项式回归方程考察pH、温度、Fe2+浓度及转速四个培养因素对Fe2+氧化速率的影响。结果显示在pH为2.25、温度为32 ℃、初始Fe2+浓度为175.36 mmol/L、转数为165 r/min时, Fe2+最高氧化速率为0.2911 g/Lh。

Leptospirillum ferrooxidans(L.f)is an extremely acidophilic and obligate autotrophy ironoxidizing bacterium. L.f plays an important role in bioleaching processes of mining industries and environmental treatment because it bears low pH and high temperature. In the course of bioleaching, the rate of metal-dissolving is correlated with the oxidation of ferrous ion. In this study, according to the method of response surface analysis with four factors including initial pH, temperature, initial concentration of Fe2+and rotation rate was adopted, which showed that pH of 2.25, temperature of 32 ℃, initial concentration of Fe2+of 175.36 mmol/L and rotation rate of 165 r/min were the best conditions. Under the optimal conditions, the forecasted maximum rate of microbial oxidation of ferrous sulfate was 0.2911 g/Lh. The simulation results are useful for practical application.