采用傅里叶变换红外光谱结合二阶导数光谱对不同产地的中国沙棘进行识别研究。 结果表明, 不同产地中国沙棘的一维红外光谱在2　925, 2　854, 1　743, 1　541和1　173 cm-1等处都有表征脂类、 黄酮类和糖类成分的特征吸收峰。 但因产地不同, 各样本吸收峰的位置和强度均存在一定差异。 此外, 3 429～3 336 cm-1范围处以及1 744 cm-1附近处的吸收峰是识别不同产地中国沙棘主要特征峰; 比较各产地中国沙棘的二阶导数红外图谱发现, 1 030和1 516 cm-1的吸收峰能够进一步确认样本中的黄酮类化合物的存在。 此外, 各样本在1 711和1 476 cm-1附近的吸收峰以及1 689～1 515和1 400～1 175 cm-1处吸收峰的强度和位置均具有显著差异。 一维红外光谱和二阶导数光谱的结合, 可以为不同产地中国沙棘的识别分析提供科学数据。 该方法快速、 直观、 简便, 能够为不同产地中国沙棘的整体化学成分提供大量信息, 有助于中国沙棘的整体质量控制以及有效成分定性分析研究。

The accurate identification of traditional Chinese medicine (TCM) which collected from different producing areas is important for its quality control and clinical effects. In the present study, Fourier transform infrared spectroscopy (FTIR) combined with second derivative spectra were used to identify and analyze H. rhamnoides subsp. sinensis from different producing areas. The characteristic absorption peaks, including 2　925, 2 854, 1 743, 1 541 and 1 173 cm-1 belonging to fatty acids, flavonoids and saccharides appear in all 20 samples. But the absorption peak intensities and locations varied due to the different geographical regions. The results also showed that the absorption peaks at the range of 3 429～3 336 and 1 744 cm-1 were important characteristic absorption peaks which can identify H. rhamnoides subsp. sinensis from different producing areas. Also, absorption peaks at 1　030 and 1　516 cm-1 further confirmed the existence of flavonoids in all samples by comparing the second derivative infrared spectra in the range of 1　800～1　000 cm-1. However, the samples’ differences can be intuitively found around peaks 1　711, 1　476 cm-1 and ranges from 1　689～1　515　and 1　400～1　175 cm-1. The results demonstrated that FTIR was a simple, convenient, fast and intuitive approach to identify and analyze H. rhamnoides subsp. sinensis from different producing areas. This method provides foundations for the analysis of chemical compositions and quality control for the TCM.