采用红外光谱、 紫外光谱、 荧光光谱及微波消解电感耦合等离子体质谱法对海南白沙和霸王岭两个产地的草豆蔻进行了分析。 其乙醇提取物的红外光谱都具有1 051, 1 390, 2 976及3 300 cm-1左右表征黄酮类成分的特征吸收峰, 二阶红外导数谱图显示了在2 977.72及2 899.94有相似吸收峰, 在1 922.36及1 650.87 cm-1有显著差异吸收峰, 可以鉴定区分海南不同产地的草豆蔻。 利用紫外光谱法表征了两个不同产地草豆蔻的特征谱图, 并根据有关的吸收原理, 建立了测定草豆蔻植物中主要组分山姜素与豆蔻明含量的方法, 获得了良好的分析精密度(0.42%～0.29%)和回收率(84.28%～117.41%)。 测得白沙草豆蔻中山姜素和豆蔻明的含量分别为4.23%和3.83%, 而霸王岭的分别为3.72%和3.34%。 荧光测定结果表明, 不同产地的草豆蔻乙醇提取物具有相似的荧光发射及激发光谱谱图, 荧光强度的差别也再次证明白沙草豆蔻乙醇提取物的浓度大于霸王岭的。 利用微波消解电感耦合等离子体质谱法分析了草豆蔻中的K, Ca, Mg, Na, Al, Fe, Mn, Cu, Zn等十七种金属元素及其含量。 该研究为定性、 定量地鉴定区别不同产地或不同种植批次的草豆蔻及测定其中主要活性组分的含量建立了简单可行的方法; 根据实验测得的金属元素种类及含量的结果, 可为进一步开发利用草豆蔻及提高食品安全意识提供更好的理论依据。

Fourier transform infrared spectroscopy (FT-IR), UV spectroscopy and fluorescence spectroscopy combined with inductively coupled plasma mass spectrometry (ICP-MS) were employed to analyze Alpinia Katsumadai harvested from Hainan Baisha and Bawangling. The results from FT-IR indicated that the emergence of several characteristic absorption peaks around 1 051, 1 390, 2 976 and 3 300 cm-1 belong to flavonoids. In light of second derivative spectra, two similar peaks around 2 977.72 and 2 899.94 cm-1 and evident differences peaks around 1 922.36 and 1 650.87 cm-1 were observed, which was obvious to identify and distinguish Hainan Alpinia Katsumadai from different geographical regions. The method of UV spectroscopy were used to determine the different characteristic spectra of Alpinia Katsumadai harvested from Hainan Baisha and Bawangling. An UV-quantitative analysis method for alpinetin and cardamonin in Alpinia Katsumadai was established according to the relevant absorption principle. The results from fluorescence experiments revealed that both ethanol extracts of Alpinia katsumadai Hayata harvestd from Baisha and Bawangling have the similar fluorescence emission spectra and excitation spectra. The difference of fluorescence intensity once again demonstrated the concentration of ethanol extracts of Alpinia katsumadai Hayata harvestd from Baisha was bigger than that of Bawangling. The good relative recovery was in the range of 84.28%～117.41% with the RSDs (n=3) of 0.42%～0.29%. The content of alpinetin and cardamomin in Alpinia katsumadai from various habitats are 4.23% and 3.83% for Baisha, 3.72% and 3.34% for Baiwangling, respectively. Seventeen kinds of metal elements including K, Ca, Mg, Na, Al, Fe, Mn, Cu, Zn et al were determined by using microwave digestion-ICP-MS. Therefore, FT-IR combined with second derivative spectra was an express and comprehensive to analyze and evaluate the subtle difference among different habitats. It was also indicated that the method of UV-absorption spectroscopy was simple and reliable for identifing Alpinia katsumadai from differern geographical regions and cultivation batches, and determining qualitatively and quantitatively their main active components. The determination results of metal elements according to ICP-MS experiments will provide a theoretical foundation for the further development and utilization Alpinia katsumadai and enhancing the food danger consciousness.