光谱学与光谱分析, 2016, 36 (6): 1706, 网络出版: 2016-12-20  

氯化钠对葡萄糖水溶液近红外光谱的影响

Study on the Effect of Sodium Chloride Salt on Near-Infrared Spectroscopy of Glucose Aqueous Solution
作者单位
1 天津大学精密测试技术及仪器国家重点实验室, 天津 300072
2 天津大学生物医学检测技术与仪器天津市重点实验室, 天津 300072
摘要
人体内钠盐的含量影响血糖代谢且与糖尿病具有较高的相关性。 因此, 在进行血糖的近红外光谱无创检测时, 不仅要考虑血液中大颗粒及大分子物质对光谱的吸收和散射影响, 也应从分子结构层面上分析小分子物质对葡萄糖分子结构及其特征吸收的影响。 基于声光可调谐滤波器(AOTF)的高精度近红外光谱采集系统, 测量并研究了在水溶液环境下氯化钠(NaCl)对葡萄糖分子结构及其近红外特征吸收的影响。 首先, 测量含有不同NaCl含量的葡萄糖水溶液透射光谱, 分别采用纯水和同浓度 NaCl 样本进行背景修正, 实验表明, 在水溶液环境中 NaCl会改变水分子和葡萄糖分子特征吸收峰的位置和强度; 对不含NaCl和含有NaCl的糖水样本分别扣除纯水和同浓度NaCl样本后进行二维相关光谱分析, 同步谱的切线谱显示NaCl减弱了葡萄糖分子在1 400和1 520~1 700 nm处的特征吸收。 最后, 通过偏最小二乘回归模型定量分析NaCl对葡萄糖预测精度的影响, 发现模型的预测均方根误差随NaCl含量的增加而增大, 并且含NaCl的样本与不含NaCl的样本对葡萄糖浓度预测值之差的平均值与加入的NaCl含量近似为线性关系。 结果表明, 在水溶液环境下NaCl分子会改变葡萄糖分子键状态并影响其特征吸收, 从而降低模型的预测精度。 若将NaCl含量作为变量因子, 有助于提升血糖的近红外光谱无创检测精度。
Abstract
The sodium chloride (NaCl) salt has been reported to be associated with glucose metabolism. However, the effect of it on non-invasive detection of blood glucose using near-infrared spectroscopy is still an open question. The aim of this study was to investigate this affection through transform background correction analysis two-dimensional (2D) correlation synchronous spectrum and the partial least-squares (PLS) regression. First, the transmittances of glucose aqueous solutions with different NaCl content are collected and the pure water and NaCl aqueous solution are measured as the background. Results show that, the dissolving of NaCl in water changes the amplitude and position of the absorption peak of water. There are two negative peaks in 1 400 and 1 500~1 700 nm corrected spectra of NaCl aqueous obviously and the amplitude of peaks associated with NaCl concentration. That’s because NaCl affect the molecular binding and vibration of water. Then the glucose aqueous solutions without NaCl and with NaCl are corrected by the spectra of pure water and NaCl aqueous solution, respectively. So we get the conclusion that NaCl also affect the combination of glucose and water molecules. And the two-dimensional correlation spectroscopy analysis is performed under the perturbation of glucose concentration. The slice spectra of synchronous correlation spectra show that, the adding of NaCl weakens the spectral variation due to glucose concentration change in the wavelength of 1 400 and 1 520~1 700 nm. Finally, the partial least square (PLS) regression models were built to quantitatively conduct the influence of NaCl on glucose prediction accuracy. Comparison results showed that, NaCl molecule in aqueous solution will deteriorate the model accuracy, where root mean square error of prediction increases with the NaCl content; the mean difference of predicted glucose concentration between models based on glucose aqueous solutions with NaCl and without NaCl, is linear with NaCl concentration in samples.

于旭耀, 白志亮, 刘蓉, 袁晶, 余辉, 王海均, 徐可欣. 氯化钠对葡萄糖水溶液近红外光谱的影响[J]. 光谱学与光谱分析, 2016, 36(6): 1706. YU Xu-yao, BAI Zhi-liang, LIU Rong, YUAN Jing, YU Hui, WANG Hai-jun, XU Ke-xin. Study on the Effect of Sodium Chloride Salt on Near-Infrared Spectroscopy of Glucose Aqueous Solution[J]. Spectroscopy and Spectral Analysis, 2016, 36(6): 1706.

关于本站 Cookie 的使用提示

中国光学期刊网使用基于 cookie 的技术来更好地为您提供各项服务,点击此处了解我们的隐私策略。 如您需继续使用本网站,请您授权我们使用本地 cookie 来保存部分信息。
全站搜索
您最值得信赖的光电行业旗舰网络服务平台!