光谱学与光谱分析, 2016, 36 (6): 1761, 网络出版: 2016-12-20
表面增强激光拉曼光谱测定豆制品中的碱性嫩黄Ⅱ、 碱性橙Ⅱ、 皂黄
Fast Determination of Auramine Ⅱ, Basic Orange Ⅱ and Metanil Yellow in Bean Products on Surface Enhanced Raman Spectroscopy and Use High Pergormance Liquid Chromatography-Tandem Mass Spectrometry to Verify
表面增强激光拉曼光谱 碱性嫩黄Ⅱ 碱性橙Ⅱ 皂黄 液相色谱-串联质谱法 Surface enhanced Raman spectroscopy Auramine Ⅱ Basic orange Ⅱ Metanil yellow High pergormance liquid chromatography-tandem mass
摘要
以表面增强激光拉曼光谱技术快速检测了豆制品中碱性嫩黄Ⅱ、 碱性橙Ⅱ和皂黄的含量, 并以高效液相色谱-串联质谱法进行了确证。 优化出最佳提取溶剂为甲醇-水(7+3)溶液, 样品前处理采用快速溶剂萃取仪(ASE)提取和凝胶渗透色谱(GPC)净化, 对ASE和GPC条件进行了优化, 提高了提取效率、 检测灵敏度、 减少提取溶剂用量并且有效去除了基质中大分子的干扰物。 对三种色素的表面增强拉曼谱中的特征峰进行了归属认证。 碱性嫩黄Ⅱ、 碱性橙Ⅱ和皂黄的定量特征峰分别为652, 995和983 cm-1; 方法检出限为3.0, 1.0和4.0 mg·kg-1。 三种色素定量特征峰与色素浓度呈良好的线性关系, 实验的回收率在83.48%~92.59%范围内, 相对标准偏差小于7.2%。 该方法新颖、 前处理、 设备操作简单、 分析速度快、 重现性好、 灵敏度高, 成功的对食品中色素进行了定性和定量测定, 为食品中色素的检测提供了可靠的参考。
Abstract
This experiment adopts Surface Enhanced Raman Spectroscopy (SERS) to quickly detect auramine Ⅱ, basic orange Ⅱ and metanil yellow in bean products. It uses High Performance Liquid Chromatography (HPLC)-tandem mass spectrometry to verify. The best extraction solvent is methanol-water (Seven plus three) solution. Before classification, extracting the bean products withAccelerated Solvent Extaction (ASE) and purifying it with Gel Permeation Chromatography(GPC), which improves the extraction efficiency, improves the detection sensitivity, reduces the dosage of extraction solvent and effective in the matrix of macromolecular distractors.ASE and GPC conditions are optimized. This study of three types of pigment surface enhanced Raman spectra characteristic peak of the ownership certification. The characteristic peak of auramine Ⅱ, basic orange Ⅱ and metanil yellow is respectively 652, 995 and 983 cm-1; he method detection limit is 3.0, 1.0 and 4.0 mg·kg-1. Three quantitative characteristic peak of pigment had a good linear relationship with pigment concentration, Recovery of this experiment was 83.48%~92.59% range, relative standard deviation less than 7.2%. The method is characterized by simple pretreatment, short analysis period and high sensitivity, etc. The method provides a reliable reference for food pigment detection.
闫正, 张丽冰, 任孟伟, 杨照生, 布雅楠, 蔡立鹏. 表面增强激光拉曼光谱测定豆制品中的碱性嫩黄Ⅱ、 碱性橙Ⅱ、 皂黄[J]. 光谱学与光谱分析, 2016, 36(6): 1761. YAN Zheng, ZHANG Li-bing, REN Meng-wei, YANG Zhao-sheng, BU Ya-nan, CAI Li-peng. Fast Determination of Auramine Ⅱ, Basic Orange Ⅱ and Metanil Yellow in Bean Products on Surface Enhanced Raman Spectroscopy and Use High Pergormance Liquid Chromatography-Tandem Mass Spectrometry to Verify[J]. Spectroscopy and Spectral Analysis, 2016, 36(6): 1761.