生物过程是一个复杂的系统, 在反应过程中, 会产生固态、 液态、 气态的过程反应物, 且具有生物活性, 想要准确的掌握过程反应物的种类与含量, 必须对生物过程进行实时在线的监测, 才能够获得准确的数据。 生物过程的原位在线分析依赖于高性能的衰减全反射探头, 衰减全反射技术是在线分析系统中实现原位在线测量的有力手段。 通过分析衰减全反射技术的基本原理与特点, 设计了一种成本低、 光通量高、 具有标准生物过程传感器接口的中红外衰减全反射探头, 能够直接插入到生物反应器中, 对生物过程反应物进行无损、 零延迟的监测。 实验结果表明, 在线式ATR探头具有较宽的光谱范围, 所采集光谱具有较高的信噪比, 能够应用在生物过程的在线定量分析之中。

The in situ biological process relays on a high performance attenuated total reflection (ATR) probe while ATR technique is a powerful tool for inline analysis. The basic principle and characteristic of the ATR technique was analyzed in this paper. A low cost and high throughout ATR probe was designed which can be inserted into the biological reactor directly with nondestructive and zero delay monitoring. It shows that the online ATR probe has a wide spectrum range, and the collected spectra has a high signal-to-noise ratio, which can be used in in-line quantitative analysis for biological process.