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多色单分子定位超分辨显微成像术

Multicolor Single-Molecule Localization Super-Resolution Microscopy

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摘要

多色成像作为超分辨成像技术的重要延伸, 极大地增强了人们研究亚细胞结构定位与交互关系的能力, 从而有助于研究者深入理解细胞内复杂的生命现象与过程。基于单分子定位超分辨显微成像术(SMLM)工作原理的特殊性, 已实现了激发依赖、激活依赖、分光依赖等数种有特点的多色成像方法。介绍6种主要的多色单分子定位超分辨显微成像技术, 从分色能力、光谱窜扰、数据采集效率等角度分析了各方法的优缺点, 并讨论了与多色成像相关的细胞固定方法, 帮助研究人员根据自身实验需求选择合适可靠的多色成像手段研究相应的科学问题。

Abstract

Multicolor imaging is an important extension of super-resolution microscopy, which greatly enhances the investigation ability of the relationship between localization and interaction of sub-cellular structures. Therefore, it is beneficial for researchers to go deep into understanding of complicated biological phenomena and processes in cells. Based on the special working principle of single-molecule localization super-resolution microscopy (SMLM), several characteristic multicolor imaging technologies including excitation-dependent, activation-dependent, split-dependent multicolor SMLM and so on are accomplished. In this paper, we analyze the advantages and disadvantages among six main multicolor SMLM from the views of color separation ability, spectrum cross-talk and data collection efficiency. In addition, we discuss the cell fixation method in relation to the multicolor imaging. This review may be helpful for researchers to seek suitable and reliable multicolor imaging methods to study corresponding scientific problem according to their own experimental conditions.

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中图分类号:Q63

DOI:10.3788/aos201737.0318010

所属栏目:“超分辨成像”专题

基金项目:国家自然科学基金(11574165)、111计划(B07013)、长江学者和创新团队发展计划(IRT_13R29)

收稿日期:2016-10-13

修改稿日期:2016-11-23

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潘雷霆:南开大学物理科学学院, 泰达应用物理研究院弱光非线性光子学教育部重点实验室, 天津 300457南开大学生物治疗协同创新中心, 天津 300457
胡芬:南开大学物理科学学院, 泰达应用物理研究院弱光非线性光子学教育部重点实验室, 天津 300457
张心正:南开大学物理科学学院, 泰达应用物理研究院弱光非线性光子学教育部重点实验室, 天津 300457山西大学极端光学协同创新中心, 山西 太原 030006
许京军:南开大学物理科学学院, 泰达应用物理研究院弱光非线性光子学教育部重点实验室, 天津 300457山西大学极端光学协同创新中心, 山西 太原 030006

联系人作者:潘雷霆(plt@nankai.edu.cn)

备注:潘雷霆(1983-), 男, 博士, 副教授, 主要从事光生物医学光子学方面的研究。

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引用该论文

Pan Leiting,Hu Fen,Zhang Xinzheng,Xu Jingjun. Multicolor Single-Molecule Localization Super-Resolution Microscopy[J]. Acta Optica Sinica, 2017, 37(3): 0318010

潘雷霆,胡芬,张心正,许京军. 多色单分子定位超分辨显微成像术[J]. 光学学报, 2017, 37(3): 0318010

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