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结构导向的可逆光激活绿色荧光蛋白探针的研制

Structure-Guided Development of Reversibly Photoswitchable Green Fluorescent Proteins Probe

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摘要

近几年, 可逆光激活荧光蛋白的研制越来越受到人们的重视, 这类荧光蛋白极大地促进了活细胞超高分辨显微成像技术的发展及应用。可逆光激活荧光蛋白可被不同波长的光多次可逆地进行调制, 因而被广泛地应用于高密度数据的光存储、光致变色荧光共振能量转移的测量以及基于可逆饱和线性荧光跃迁原理的超高分辨率显微成像中。从研制这类荧光蛋白所涉及的关键氨基酸位点出发, 本文综述了近几年可逆光激活绿色荧光蛋白的研制进展, 并简要地讨论荧光蛋白结构与光学特性的关系, 从而为后续结构导向的可逆光激活荧光蛋白的研制提供参考。

Abstract

In recent years, people pay more and more attentions to the development of reversibly photoswitchable fluorescent proteins (RSFPs), because those fluorescent proteins can greatly promote the development and application of live-cell super-resolution imaging techniques. Reversibly photoswitchable fluorescent protein can be repeatedly and reversibly modulated by different wavelength lights, thus it can be widely applied in many fields such as high density data optical storage, the measurement of photochromic fluorescence resonance energy transfer, and super-resolution imaging based on reversible saturable linear fluorescence transitions (RESOLFT) principle. The recent development of green RSFPs is reviewed from the key amino acids involved in RSFPs design. We also make a brief discussion of the relationship between structure and optical characteristics of RSFPs, serving as the reference for further structure-guided development of better RSFPs.

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中图分类号:Q336

DOI:10.3788/aos201737.0318002

所属栏目:“超分辨成像”专题

基金项目:国家自然科学基金(21573013,21390412,31271423,31327901)、国家863计划(SS2015AA020406)

收稿日期:2016-10-31

修改稿日期:2016-12-28

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王盛:北京大学生物膜与膜生物工程国家重点实验室, 北京 100871北京大学生物动态光学成像中心, 北京 100871北京大学生命科学学院, 北京 100871
陈轩泽:北京大学生物膜与膜生物工程国家重点实验室, 北京 100871北京大学生物动态光学成像中心, 北京 100871北京大学生命科学学院, 北京 100871
常蕾:北京大学生物膜与膜生物工程国家重点实验室, 北京 100871北京大学生物动态光学成像中心, 北京 100871北京大学生命科学学院, 北京 100871
薛瑞莹:北京大学生物膜与膜生物工程国家重点实验室, 北京 100871北京大学生物动态光学成像中心, 北京 100871北京大学生命科学学院, 北京 100871
孙育杰:北京大学生物膜与膜生物工程国家重点实验室, 北京 100871北京大学生物动态光学成像中心, 北京 100871北京大学生命科学学院, 北京 100871

联系人作者:王盛(wangsheng9411@sina.com)

备注:王盛(1981-), 男, 博士后, 助理研究员, 主要从事超分辨显微成像技术方面的研究。

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引用该论文

Wang Sheng,Chen Xuanze,Chang Lei,Xue Ruiying,Sun Yujie. Structure-Guided Development of Reversibly Photoswitchable Green Fluorescent Proteins Probe[J]. Acta Optica Sinica, 2017, 37(3): 0318002

王盛,陈轩泽,常蕾,薛瑞莹,孙育杰. 结构导向的可逆光激活绿色荧光蛋白探针的研制[J]. 光学学报, 2017, 37(3): 0318002

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