超分辨显微成像技术使细胞生物学进入到了一个全新的时代, 但如何进一步提高超分辨显微成像技术的时空分辨率仍是光学领域需要解决的重要问题。目前为止几乎所有的超分辨显微成像技术都依赖于荧光探针, 光调控荧光蛋白作为一类特殊的荧光探针, 可以被不同波长的激发光所激活, 产生随机或者特殊结构样式的信号。利用这些信息, 透镜系统的空间分辨率得到了提高。通过总结光调控荧光蛋白的各类参数, 从荧光探针入手, 寻找进一步提高成像系统空间分辨率的方法与策略, 为选取适当的荧光探针提供建议, 并且阐述了荧光蛋白与超分辨显微成像技术之间的关系。

Cell biology has come into a new era by the hand of super-resolution microscopy. However, how to improve the temporal-spatial resolution of super-resolution microscopy is still an important problem to be resolved in the field of optics. So far, almost all of the super-resolution microscopies are depend on fluorescent probes. As a unique series of fluorescent probes, light regulated fluorescent proteins can be activated by excitation light of different wavelengths to produce stochastic or specially patterned signals. Using this information, the spatial resolution of lens system is improved. By summarizing different parameters of light regulated fluorescent proteins, we focus on fluorescent probes to discuss the way of improving spatial resolution of imaging system. It provides advices to choose proper fluorescent probes, and the relationship between fluorescent proteins and super-resolution microscopy is expounded.