中国激光, 2017, 44 (3): 0309001, 网络出版: 2017-03-08
基于迈克耳孙干涉仪的非相干数字全息显微成像
Incoherent Digital Holographic Microscopic Imaging Based on Michelson Interferometer
全息 非相干数字全息 显微成像 广义相移数字全息干涉术 holography incoherent digital holography microscopic imaging generalized phase-shifting digital holographic int
摘要
采用基于迈克耳孙干涉仪的非相干数字全息显微成像系统能够得到物体在非相干光照明下的全息图。对基于迈克耳孙干涉仪的非相干数字全息显微成像系统进行了理论和实验研究。利用标量衍射理论计算了该系统在记录过程中的点扩展函数,获得了系统横向放大率及重建距离的具体表达式。搭建了基于迈克耳孙干涉仪的非相干数字全息显微成像系统的实验光路,利用CCD记录全息图,用广义相移数字全息干涉术去除孪生像与零级像,并用角谱算法得到了清晰的重建像。实现了分辨率板和洋葱表皮细胞等样品的非相干全息显微成像,验证了该系统的可行性。分辨率板的成像实验表明,该系统的横向分辨率可达512 lp/mm。微米洁面刷软毛的成像实验表明,该系统具有呈现物体三维结构的特性。
Abstract
The incoherent digital holographic microscopic imaging system based on the Michelson interferometer can be used to obtain the hologram of object under illumination of incoherent light. The incoherent digital holographic microscopic imaging system based on the Michelson interferometer is studied theoretically and experimentally. The point spread function of the system in the recording process has been calculated according to the scalar diffraction theory, and the specific expressions of lateral magnification and reconstruction distance of the system are deduced. A experimental light path of incoherent digital holographic microscopic imaging system based on the Michelson interferometer is built, and a CCD is used to record the holograms. A clear reconstruction image is obtained without conjugate image and zero-order image by the generalized phase-shifting digital holographic interferometry and via the angular spectrum algorithm. Furthermore, the incoherent holographic microscopic imaging of a resolution board and onion epidermal cells have been implemented, which proves the feasibility of the system. The imaging experiment of the resolution board shows that a lateral resolution as high as 512 lp/mm can be obtained. The system is able to present the characteristics of the three-dimensional structure of objects, which is verified by the imaging of the micron cleansing brush fur.
张文斌, 刘亚飞, 李德阳, 马凤英, 弓巧侠, 杜艳丽. 基于迈克耳孙干涉仪的非相干数字全息显微成像[J]. 中国激光, 2017, 44(3): 0309001. Zhang Wenbin, Liu Yafei, Li Deyang, Ma Fengying, Gong Qiaoxia, Du Yanli. Incoherent Digital Holographic Microscopic Imaging Based on Michelson Interferometer[J]. Chinese Journal of Lasers, 2017, 44(3): 0309001.