光子学报, 2017, 46 (3): 0312001, 网络出版: 2017-04-10  

MicroRNA快速检测系统的设计

Design of a Rapid MicroRNA Detection System
作者单位
1 中国科学院合肥物质科学研究院 医学物理与技术中心, 合肥 230031
2 中国科学技术大学, 合肥 230026
3 中国医科大学, 沈阳 110001
摘要
为了实现MicroRNA的快速检测, 设计了一种便携式MicroRNA快速检测仪.基于等温滚环扩增技术, 采用光电检测方法, 检测标志物受激发出的荧光光强, 建立特征荧光分析检测系统.通过改变激发光强度、MicroRNA试剂浓度等参量, 验证了该仪器可测量的MicroRNA的浓度范围为0.01~0.1 μmol, 可检测出的最低检出限为7个拷贝数, MicroRNA浓度与荧光信号强度之间为线性关系(R2=0.999 1).
Abstract
Using Opto-electronic detection technology, marking miRNA and isothermal rolling circle amplification and detecting markers which excite fluorescence intensity, we have designed a new portable device for the rapid detection of miRNA, the recognition system of characteristic fluorescence analysis was established eventually. we have got the point that there is the linear relationship (R2=0.999 1) between miRNA concentration and fluorescence intensity at the time that the miRNA concentration range the instrument can measure is in the range of 0.01~0.1 μmol by changing the excitation light intensity, miRNA reagent concentration and other parameters, meanwhile, we also know that it is 7 copies that the lowest values the instrument can measure in theory.

王燕飞, 余东升, 陈海燕, 张战盈, 方蔚恺, 陆泽橼, 李艳蕾, 纪玉峰, 关一夫, 徐赤东, 江海河. MicroRNA快速检测系统的设计[J]. 光子学报, 2017, 46(3): 0312001. WANG Yan-fei, YU Dong-sheng, CHEN Hai-yan, ZHANG Zhan-ying, FANG Wei-kai, LU Ze-yuan, LI Yan-lei, JI Yu-feng, GUAN Yi-fu, XU Chi-dong, JIANG Hai-he. Design of a Rapid MicroRNA Detection System[J]. ACTA PHOTONICA SINICA, 2017, 46(3): 0312001.

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