激光生物学报, 2017, 26 (3): 224, 网络出版: 2017-07-07   

基于CdSe掺杂锐钛矿TiO2的可见光体外灭活HL60细胞实验研究

An Experimental Study on the Visible Light Inactivated HL60 Cells in vitro Based on CdSe Doped Anatase TiO2
作者单位
广东省量子调控与材料重点实验室, 华南师范大学物理与电信工程学院, 广东 广州 510006
摘要
用超声驱动方法合成了CdSe/TiO2复合纳米粒子, 并通过扫描电镜(SEM)、透射电镜(TEM)、X射线衍射(XRD)、紫外可见光(UV-Vis)吸收光谱和荧光(FS)光谱对CdSe/TiO2复合纳米粒子进行表征。使用CCK-8法测定CdSe/TiO2对白血病细胞的可见光光催化活性并通过SEM研究HL60细胞的表面超微结构形态。实验结果表明, 用CdSe/TiO2复合纳米粒子处理的组中观察到明显的HL60细胞生长抑制, 并且HL60细胞在CdSe掺杂TiO2复合纳米粒子作用下的PDT效率显著高于TiO2, 表明可以通过CdSe的修饰有效增强TiO2的可见光光催化活性。此外, CdSe/TiO2在可见光辐照下在4 μg/mL的终值浓度下显示非常高的光动力效率, 达76%。荧光光谱分析表明, CdSe/TiO2复合纳米粒子可能通过分离光生空穴电子对提高此复合纳米粒子的光催化活性, 从而提高CdSe/TiO2对HL60细胞的PDT灭活效率。
Abstract
CdSe/TiO2 composite nanoparticles were synthesized by ultrasound-driven approach and characterized by SEM, TEM, XRD, UV-Vis and FS.The visible light photocatalytic activities of CdSe/TiO2 on leukemia HL60 cells were investigated by CCK-8 approach and the ultrastructural morphology of HL60 cells was also studied by SEM.The experimental results indicated that an obvious inhibition of leukemia HL60 cells growth would be observed in groups treated with CdSe/TiO2 composite nanoparticles, and the PDT effiency in the presence of CdSe doped TiO2 was significantly higher than that of TiO2, revealing that the visible light photocatalytic activities of TiO2 could be efficiency enhanced by the modification of CdSe.Additionally, CdSe/TiO2 can exhibit a very high photodynamic efficiency of 76% at a final indicated concentration of 4 μg/mL under visible light irradiation.The fluorescence spectrum analysis shows that the CdSe/TiO2 composite nanoparticles could improve the photocatalytic activity of the composite nanoparticles by separating the photo-generated hole pairs, thus enhancing the PDT inactivation efficiency of HL60 cells.

薛婷, 郭文博, 梁雪影, 罗丹, 艾保全, 熊建文. 基于CdSe掺杂锐钛矿TiO2的可见光体外灭活HL60细胞实验研究[J]. 激光生物学报, 2017, 26(3): 224. XUE Ting, GUO Wenbo, LIANG Xueying, LUO Dan, AI Baoquan, XIONG Jianwen. An Experimental Study on the Visible Light Inactivated HL60 Cells in vitro Based on CdSe Doped Anatase TiO2[J]. Acta Laser Biology Sinica, 2017, 26(3): 224.

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