Chinese Optics Letters, 2017, 15 (9): 090006, Published Online: Jul. 19, 2018
Implementation of FLIM and SIFT for improved intraoperative delineation of glioblastoma margin Download: 880次
170.3650 Lifetime-based sensing 170.3880 Medical and biological imaging 170.4580 Optical diagnostics for medicine
Abstract
The aim of this study is to develop a novel technique for improving the intraoperative margin assessment of glioblastoma by examining the total extrinsic extracellular matrix (ECM) with eosin staining using fluorescence lifetime imaging microscopy (FLIM) and scale-invariant feature transform (SIFT) descriptor analysis. Pseudo-color FLIM images obviously exhibit ECM distributions, changes in sequential sections, and different regions of interest. Meanwhile, SIFT descriptors are first utilized for the discrimination of glioblastoma margins by matching similar ECM regions and extracting keypoint orientations from FLIM images obtained from a series of continuous slices. The findings indicate that FLIM imaging with SIFT analysis of the total ECM is a promising method for improving intraoperative diagnosis of frozen and surgically excised brain specimen sections.
Danying Lin, Teng Luo, Liwei Liu, Yuan Lu, Shaoxiong Liu, Zhen Yuan, Junle Qu. Implementation of FLIM and SIFT for improved intraoperative delineation of glioblastoma margin[J]. Chinese Optics Letters, 2017, 15(9): 090006.