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基于光脉冲诱导快相与弛豫荧光的光合作用参数测量技术

Photosynthesis Parameters Measurement Technology Based on Fast Phase and Relaxation Fluorescence Induced by Optical Pulses

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摘要

为了更准确获取反映植物生理状态的荧光动力学曲线,基于光合作用电子传递过程研究了植物光合作用参数测量技术。采用可变光脉冲技术将植物光合作用过程分段为快相与弛豫过程,并测量激发光诱导产生的荧光动力学曲线.对激发光带宽与响应时间进行了定量分析;对I-V转换单元与MFB滤波器进行了设计与仿真分析,获取快相荧光动力学信息;采用同步脉冲采样积分技术,对微弱弛豫荧光进行积分,实现了快相与弛豫荧光动力学曲线的完整测量,并结合非线性拟合算法获取光合作用参数.测试结果表明,系统信噪比达到23.8 dB;暗适应与光适应下,本系统所测Fv/Fm与Water-PAM测量结果的线性相关系数分别达到0.980和0.997.该研究结果为植物光合作用研究及过程参数测量提供了一种测量手段.

Abstract

The photosynthetic parameters can reflect the physiological state of plants. In order to obtain a more accurate fluorescence kinetic curve, a technique for measuring plant photosynthetic parameters based on the electron transfer process was proposed in this paper. Variable excitation light pulses was employed to devide the plant photosynthesis process into two stages: fast phase and relaxation phase, and corresponding fluorescence curves were induced for each stage. The excitation bandwidth and response time were analyzed using the TINA simulation tool. The I-V conversion and MFB filter were designed and simulated quantitatively. Synchronous sampling integral technique was employed to improve the SNR of weak relaxation phase fluorescence. As a result, a complete fluorescence kinetics curve that consist of fast phase and relaxation phase was obtained, from which the plant photosynthesis parameters can be calculated using nonlinear fitting algorithm. Experiment results showed that the system SNR reached 23.8 dB, and the correlation coefficients of measured Fv/Fm that obtained by this system were respectively 0.980 and 0.997 for dark and light adaptation conditions.

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中图分类号:X835

DOI:10.3788/gzxb20174609.0930003

基金项目:The National Natural Science Foundation of China (No. 31400317), the National Laboratory Open Program for Marine Science and Technology of Qingdao (No.QNLM2016ORP0312), the National High Technology Research and Development Program of China(No. 2014AA06A509), the National Key Research and Development Programof China (No. 2016YFC1400602) and the Science and Technology Major SpecialProject of Anhui Province (No. 15CZZ04125), Natural Science Foundation of Anhui Province (No.1708085QD87), STS Program of Chinese Academy of Science (No.KFJ-SW-STS-170)

收稿日期:2017-02-24

修改稿日期:2017-06-19

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覃志松:中国科学院环境光学与技术重点实验室安徽光学精密机械研究所,合肥 230031中国科学技术大学,合肥 230026桂林电子科技大学,广西 桂林 541004安徽省环境光学监测技术重点实验室,合肥230031
殷高方:中国科学院环境光学与技术重点实验室安徽光学精密机械研究所,合肥 230031安徽省环境光学监测技术重点实验室,合肥230031
赵南京:中国科学院环境光学与技术重点实验室安徽光学精密机械研究所,合肥 230031安徽省环境光学监测技术重点实验室,合肥230031
石朝毅:合肥学院,合肥 230601
甘婷婷:中国科学院环境光学与技术重点实验室安徽光学精密机械研究所,合肥 230031安徽省环境光学监测技术重点实验室,合肥230031
肖雪:中国科学院环境光学与技术重点实验室安徽光学精密机械研究所,合肥 230031安徽省环境光学监测技术重点实验室,合肥230031
段静波:中国科学院环境光学与技术重点实验室安徽光学精密机械研究所,合肥 230031安徽省环境光学监测技术重点实验室,合肥230031
张小玲:中国科学院环境光学与技术重点实验室安徽光学精密机械研究所,合肥 230031中国科学技术大学,合肥 230026安徽省环境光学监测技术重点实验室,合肥230031
陈双:中国科学院环境光学与技术重点实验室安徽光学精密机械研究所,合肥 230031中国科学技术大学,合肥 230026安徽省环境光学监测技术重点实验室,合肥230031
刘建国:中国科学院环境光学与技术重点实验室安徽光学精密机械研究所,合肥 230031安徽省环境光学监测技术重点实验室,合肥230031
刘文清:中国科学院环境光学与技术重点实验室安徽光学精密机械研究所,合肥 230031安徽省环境光学监测技术重点实验室,合肥230031

联系人作者:覃志松(zsqin@aiofm.ac.cn)

备注:QIN Zhi-song(1977-), male, lecturer, Ph.D. candidate, mainly focuses on fluorescence detection and analysis technology.

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引用该论文

QIN Zhi-song,YIN Gao-fang,ZHAO Nan-jing,SHI Chao-yi,GAN Ting-ting,XIAO Xue,DUAN Jing-bo,ZHANG Xiao-ling,CHEN-Shuang,LIU Jian-guo,LIU Wen-qing. Photosynthesis Parameters Measurement Technology Based on Fast Phase and Relaxation Fluorescence Induced by Optical Pulses[J]. ACTA PHOTONICA SINICA, 2017, 46(9): 0930003

覃志松,殷高方,赵南京,石朝毅,甘婷婷,肖雪,段静波,张小玲,陈双,刘建国,刘文清. 基于光脉冲诱导快相与弛豫荧光的光合作用参数测量技术[J]. 光子学报, 2017, 46(9): 0930003

被引情况

【1】张小玲,殷高方,赵南京,杨瑞芳,覃志松,陈双,甘婷婷,肖雪,段静波,刘建国,刘文清. 基于加权平均法的活体藻类三维荧光标准光谱构建. 光学学报, 2018, 38(7): 730001--1

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