为了实现以水凝胶为材料的细胞支架快速加工, 将计算全息法引入传统的飞秒激光双光子加工中, 并对全息图的生成方法和全息图对加工结构的影响进行了研究。首先, 根据贝塞尔光波动方程和其透射函数生成贝塞尔光束全息图, 分析两种参数对环形结构大小和质量的影响。然后利用生成的全息图加工得到壁厚800 nm、直径为8~15 μm不等的水凝胶（PEGDA）圆管结构。最后, 实现了基于圆管道的水凝胶细胞支架高效快速加工, 支架中圆管道壁厚800 nm、直径为8 μm。本文首次将飞秒激光全息加工技术应用于水凝胶三维支架加工, 解决了飞秒激光单点加工效率的问题。该技术在生物医学中具有广阔的应用前景。

In order to achieve rapid fabrication of hydrogel cell scaffolds, the computer-aided holographic method was employed into the traditional femtosecond laser two-photon processing. The generation of hologram as well as the influence of its parameters on the processed cylinder microstructure was studied. Firstly, Ring-shaped Bessel-beam holograms were generated according to the Bessel light wave equation and its transmission function, of which the parameters effect on the size and quality of ring structure was analyzed. Then the generated holograms were used to process tubes with a thickness of 0.8 μm and diameters from 8 μm to 15 μm in the polyethylene glycol double acrylate (PEGDA) hydrogel. Finally, an efficient and rapid processing of hydrogel cell scaffolds was realized experimentally and the diameter of hydrogel tubes was 8 μm. This technique can remarkably improve the efficiency of femtosecond laser processing, thus has great potential in many applications such as biological culture and chemical analyses.